Prematurely condensed chromosome fragments in human lymphocytes induced by high doses of high-linear-energy-transfer irradiation
文献类型：期刊论文
| 作者 | Wang, Z. Z.1; Li, W. J.1; Zhi, D. J.2; Gao, Q. X.2; Qu, Y.1 ; Wang, B. Q.1
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| 刊名 | MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
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| 出版日期 | 2009-09-01 |
| 卷号 | 679期号:1-2页码:9-12 |
| 关键词 | G2-prematurely condensed chromosome fragments Carbon ions High-dose radiation Calyculin A Biodosimeter |
| ISSN号 | 1383-5718 |
| DOI | 10.1016/j.mrgentox.2009.08.001 |
| 英文摘要 | This study provides a useful biodosimetry protocol for radiation accidents that involve high doses of heavy particle radiation. Human peripheral blood lymphocytes (PBLs) were irradiated in vitro with high doses (5-50 Gy) of charged heavy-ion particles (carbon ions, at an effective linear-energy-transfer (LET) of 34.6 keV/mu m), and were then stimulated to obtain dividing cells. PBLs were treated with 100 nM calyculin A to force chromosomes to condense prematurely, and chromosome spreads were obtained and stained with Giemsa. The G2 prematurely condensed chromosome (G2-PCC) index and the number of G2-PCC including fragments (G2-PCC-Fs) per cell for each radiation dose point were scored. Dose-effect relationships were obtained by plotting the G2-PCC indices or G2-PCC-Fs numbers against radiation doses. The G2-PCC index was greater than 5% up to doses of 15 Gy; even after a 30 Gy radiation dose, the index was 1 to 2%. At doses higher than 30 Gy, however, the G2-PCC indices were close to zero. The number of G2-PCC-Fs increased steeply for radiation doses up to 30 Gy at a rate of 1.07 Gy(-1). At doses higher than 30 Gy, the numbers of G2-PCC-Fs could not be accurately indexed because of the limited numbers of cells for analysis. Therefore, the number of G2-PCC-Fs could be used to estimate radiation doses up to 30 Gy. In addition, a G2-PCC index close to zero could be used as an indicator for radiation doses greater than 40 Gy. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS关键词 | SIMPLE BIODOSIMETRY METHOD ; RADIATION-EXPOSURE ; PCC ; ABERRATIONS ; INDUCTION ; DAMAGE ; GAMMA ; CELLS |
| 资助项目 | Chinese Academy of Sciences ; National Nature Science Foundation of China[10875153] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000272069600002 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | Chinese Academy of Sciences ; National Nature Science Foundation of China |
| 公开日期 | 2010-10-29 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/5373]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） 近代物理研究所_生物物理研究室 |
| 通讯作者 | Wang, Z. Z. |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Radiobiol Lab, Lanzhou 730000, Gansu, Peoples R China 2.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Z. Z.,Li, W. J.,Zhi, D. J.,et al. Prematurely condensed chromosome fragments in human lymphocytes induced by high doses of high-linear-energy-transfer irradiation[J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,2009,679(1-2):9-12. |
| APA | Wang, Z. Z.,Li, W. J.,Zhi, D. J.,Gao, Q. X.,Qu, Y.,&Wang, B. Q..(2009).Prematurely condensed chromosome fragments in human lymphocytes induced by high doses of high-linear-energy-transfer irradiation.MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,679(1-2),9-12. |
| MLA | Wang, Z. Z.,et al."Prematurely condensed chromosome fragments in human lymphocytes induced by high doses of high-linear-energy-transfer irradiation".MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 679.1-2(2009):9-12. |
入库方式： OAI收割
来源：近代物理研究所
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