Linear energy transfer dependence of the effects of carbon ion beams on adventitious shoot regeneration from in vitro leaf explants of Saintpaulia ionahta
文献类型：期刊论文
| 作者 | Leloup, Corinne; Zhou, Libin ; Li, Wenjian; Yu, Lixia; Li, Ping; Li, Qiang; Ma, Shuang; Dong, Xicun; Zhou, Guangming
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| 刊名 | INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
 |
| 出版日期 | 2006-07-01 |
| 卷号 | 82期号:7页码:473-481 |
| 关键词 | linear energy transfer relative biological effectiveness tissue culture carbon ion beams irradiation |
| ISSN号 | 0955-3002 |
| DOI | 10.1080/09553000600863080 |
| 英文摘要 | Purpose: To determine the effects of carbon ion beams with five different linear energy transfer (LET) values on adventitious shoots from in vitro leaf explants of Saintpaulia ionahta Mauve cultivar with regard to tissue increase, shoots differentiation and morphology changes in the shoots. Materials and methods: In vitro leaf explant samples were irradiated with carbon ion beams with LET values in the range of 31 similar to 151 keV/mu m or 8 MeV of X-rays (LET 0.2 keV/mu m) at different doses. Fresh weight increase, surviving fraction and percentage of the explants with regenerated malformed shoots in all the irradiated leaf explants were statistically analysed. Results: The fresh weight increase (FWI) and surviving fraction (SF) decreased dramatically with increasing LET at the same doses. In addition, malformed shoots, including curliness, carnification, nicks and chlorophyll deficiency, occurred in both carbon ion beam and X-ray irradiations. The induction frequency with the former, however, was far more than that with the X-rays. Conclusions: This work demonstrated the LET dependence of the relative biological effectiveness (RBE) of tissue culture of Saintpaulia ionahta according to 50% FWI and 50% SF. After irradiating leaf explants with 5 Gy of a 221 MeV carbon ion beam having a LET value of 96 keV/mu m throughout the sample, a chlorophyll-deficient (CD) mutant, which could transmit the character of chlorophyll deficiency to its progeny through three continuous tissue culture cycles, and plantlets with other malformations were obtained. |
| WOS关键词 | DOUBLE-STRAND BREAKS ; HEAVY-IONS ; ARABIDOPSIS-THALIANA ; CHROMOSOME-ABERRATIONS ; DNA ; RADIATION ; CELLS ; IRRADIATION ; MUTATIONS ; REPAIR |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Nuclear Science & Technology ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000239447200003 |
| 出版者 | TAYLOR & FRANCIS LTD |
| 公开日期 | 2010-10-29 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/5499]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） 近代物理研究所_生物物理研究室 |
| 通讯作者 | Zhou, Libin |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Columbia Univ, Ctr Radiol Res Phys & Surg, New York, NY USA |
| 推荐引用方式 GB/T 7714 | Leloup, Corinne,Zhou, Libin,Li, Wenjian,et al. Linear energy transfer dependence of the effects of carbon ion beams on adventitious shoot regeneration from in vitro leaf explants of Saintpaulia ionahta[J]. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,2006,82(7):473-481. |
| APA | Leloup, Corinne.,Zhou, Libin.,Li, Wenjian.,Yu, Lixia.,Li, Ping.,...&Zhou, Guangming.(2006).Linear energy transfer dependence of the effects of carbon ion beams on adventitious shoot regeneration from in vitro leaf explants of Saintpaulia ionahta.INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,82(7),473-481. |
| MLA | Leloup, Corinne,et al."Linear energy transfer dependence of the effects of carbon ion beams on adventitious shoot regeneration from in vitro leaf explants of Saintpaulia ionahta".INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 82.7(2006):473-481. |
入库方式： OAI收割
来源：近代物理研究所
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