Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification
文献类型：期刊论文
| 作者 | Chen, Hao-tai1; Zhang, Jie1; Sun, De-hui1; Chu, Yue-feng1; Cai, Xue-peng1; Liu, Xiang-tao1; Luo, Xue-nong1; Liu, Qing2; Liu, Yong-sheng1 |
| 刊名 | JOURNAL OF VIROLOGICAL METHODS
 |
| 出版日期 | 2008-05-01 |
| 卷号 | 149期号:2页码:264-268 |
| 关键词 | porcine circovirus type 2 (PCV2) detection loop-mediated isothermal amplification (LAMP) sensitivity specificity |
| ISSN号 | 0166-0934 |
| DOI | 10.1016/j.jviromet.2008.01.023 |
| 英文摘要 | A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). The amplification could be finished in 60 min under isothermal condition at 64 degrees C by employing a set of four primers targeting the cap gene of PCV2. The LAMP assay showed higher sensitivity than the conventional PCR, with a detection limit of five copies per tube of purified PCV2 genomic DNA. No cross-reactivity was observed from the samples of other related viruses including porcine circovirus type 1 (PCV1), porcine parvovirus (PPV), porcine pseudorabies virus (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV). The detection rate of PCV2 LAMP for 86 clinical samples was 96.5% and appeared greater than that of the PCR method. The LAMP assay reported can provide a rapid yet simple test of PCV2 suitable for laboratory diagnosis and pen-side detection due to ease of operation and the requirement of only a regular water bath or heat block for the reaction. (c) 2008 Elsevier B.V. All rights reserved. |
| WOS关键词 | MULTISYSTEMIC WASTING SYNDROME ; REAL-TIME PCR ; RESPIRATORY SYNDROME VIRUS ; EXPERIMENTAL INOCULATION ; CONVENTIONAL PIGS ; DISEASE VIRUS ; SYNDROME PMWS ; ORF2 PROTEIN ; PARVOVIRUS ; INFECTION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000255622400010 |
| 出版者 | ELSEVIER SCIENCE BV |
| 公开日期 | 2010-10-29 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/5539]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） |
| 通讯作者 | Liu, Yong-sheng |
| 作者单位 | 1.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Dept Virol,Minist Agr, State Key Lab Vet Etiol Biol,Key Lab Anim Virol, Lanzhou 730046, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730046, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Hao-tai,Zhang, Jie,Sun, De-hui,et al. Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification[J]. JOURNAL OF VIROLOGICAL METHODS,2008,149(2):264-268. |
| APA | Chen, Hao-tai.,Zhang, Jie.,Sun, De-hui.,Chu, Yue-feng.,Cai, Xue-peng.,...&Liu, Yong-sheng.(2008).Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification.JOURNAL OF VIROLOGICAL METHODS,149(2),264-268. |
| MLA | Chen, Hao-tai,et al."Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification".JOURNAL OF VIROLOGICAL METHODS 149.2(2008):264-268. |
入库方式： OAI收割
来源：近代物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。