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Induction of cytogenetic adaptive response in spermatogonia and spermatocytes by pre-exposure of mouse testis to low-dose C-12(6+) ions

文献类型：期刊论文

作者Zhang, Hong2; Zhao, Weiping1,2; Wang, Yanling1,2; Li, Ning1,2; Wu, Zhenhua2; Liu, Yang2; Chen, Jidong1,2; Cai, Yujia1,2
刊名MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
出版日期2008-05-31
卷号653期号:1-2页码:109-112
关键词C-12(6+) heavy ion low-dose pre-exposure spermatogonium and spermatocyte cytogenetic adaptive response poly(ADP-ribose) polymerase 1 (PARP-1)
ISSN号1383-5718
DOI10.1016/j.mrgentox.2008.04.001
英文摘要To investigate the effects of pre-exposure of mouse testis to low-dose C-12(6+) ions on cytogenetics of spermatogonia and spermatocytes induced by subsequent high-dose irradiation. the testes of outbred Kun-Ming strain mice were irradiated with 0.05 Gy of C-12(6+) ions as the pre-exposure dose, and then irradiated with 2 Gy as challenging dose at 4 h after per-exposure. Poly(ADP-ribose) polymerase (PARPs) activity and PARP-1 protein expression were respectively measured by using the enzymatic and Western blot assays at 4 h after irradiation; chromosomal aberrations in spermatogonia and spermatocytes were analyzed by the air-drying method at 8 h after irradiation. The results showed that there was a significant increase in the frequency of chromosomal aberrations and significant reductions of PARP activity and PARP-1 expression level in the mouse testes irradiated with 2 Gy of C-12(6+) ions. However, pre-exposure of mouse testes to a low dose of C-12(6+) ions significantly increased PARPs activity and PARP-1 expression and alleviated the harmful effects induced by a subsequent high-dose irradiation. PARP activity inhibitor 3-aminobenzamide (3-AB) treatment blocked the effects of PARP-1 on cytogenetic adaptive response induced by low-dose C-12(6+) ion irradiation. The data suggest that pre-exposure of testes to a low dose of heavy ions can induce cytogenetic adaptive response to subsequent high-dose irradiation. The increase of PARP-1 protein induced by the low-dose ionizing irradiation may be involved in the mechanism of these observations. (C) 2008 Elsevier B.V. All rights reserved.
WOS关键词DOUBLE-STRAND BREAKS ; HIGH-LET RADIATIONS ; RAT GERMINAL CELLS ; HUMAN-LYMPHOCYTES ; IONIZING-RADIATION ; OXIDATIVE STRESS ; MAMMALIAN-CELLS ; X-IRRADIATION ; DNA ; REPAIR
WOS研究方向Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
语种英语
WOS记录号WOS:000257638700015
出版者ELSEVIER SCIENCE BV
公开日期2010-10-29
源URL[http://ir.imp.cas.cn/handle/113462/5683]  
专题近代物理研究所_近代物理研究所知识存储（2010之前）
通讯作者Zhang, Hong
作者单位1.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
2.Chinese Acad Sci, Inst Modern Phys, Dept Med Phys, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Hong,Zhao, Weiping,Wang, Yanling,et al. Induction of cytogenetic adaptive response in spermatogonia and spermatocytes by pre-exposure of mouse testis to low-dose C-12(6+) ions[J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,2008,653(1-2):109-112.
APA Zhang, Hong.,Zhao, Weiping.,Wang, Yanling.,Li, Ning.,Wu, Zhenhua.,...&Cai, Yujia.(2008).Induction of cytogenetic adaptive response in spermatogonia and spermatocytes by pre-exposure of mouse testis to low-dose C-12(6+) ions.MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,653(1-2),109-112.
MLA Zhang, Hong,et al."Induction of cytogenetic adaptive response in spermatogonia and spermatocytes by pre-exposure of mouse testis to low-dose C-12(6+) ions".MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 653.1-2(2008):109-112.

入库方式： OAI收割

来源：近代物理研究所

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