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Pre-irradiation with low-dose C-12(6+) beam significantly enhances the efficacy of AdCMV-p53 gene therapy in human non-small lung cancer

文献类型：期刊论文

作者Liu Bing; Zhang Hong; Li WenJian; Li Qiang; Zhou GuangMing; Xie Yi; Hao JiFang; Min FengLing; Zhou QingMing; Duan Xin
刊名SCIENCE IN CHINA SERIES G-PHYSICS MECHANICS & ASTRONOMY
出版日期2007-04-01
卷号50期号:2页码:221-230
关键词heavy-ion beam gene therapy p53 non-small lung cancer adenoviral vector low dose
ISSN号1672-1799
DOI10.1007/s11433-007-0012-3
英文摘要The combination of ionizing radiation and gene therapy has been investigated. However, there are very few reports about the combination of heavy-ion irradiation and gene therapy. To determine if the pre-exposure to low-dose heavy ion beam enhances the suppression of AdCMV-p53 on non-small lung cancer (NSLC), the cells pre-irradiated or non-irradiated were infected with 20, 40 MOI of AdCMV-p53. Survival fraction and the relative biology effect (RBE) were determined by clonogenic assay. The results showed that the proportions of p53 positive cells in C-12(6+) beam induced AdCMV-p53 infected cells were more than 90%, which were significantly more than those in gamma-ray induced AdCMV-p53 infected cells. The pre-exposure to low-dose 12C6+ beam significantly prevented the G(0)/G(1) arrest and activated G(2)/M checkpoints. The pre-exposure to C-12(6+) beam significantly improved cell to apoptosis. RBEs for the C-12(6+)+ AdCMV-p53 infection groups were 30%-60%,20% -130% and 30%-70% more than those for the C-12(6+)_irradiated only, AdCMV-p53 infected only, and gamma-irradiation induced AdCMVp53 infected groups, respectively. The data suggested that the pre-exposure to low-dose C-12(6+) beam significantly promotes exogenous p53 expression in NSLC, and the suppression of AdCMV-p53 gene therapy on NSLC.
WOS关键词WILD-TYPE P53 ; HIGH-LET RADIATION ; SQUAMOUS-CELL CARCINOMA ; IONIZING-RADIATION ; DNA-DAMAGE ; RADIOTHERAPY ; APOPTOSIS ; GROWTH ; CARBON ; LINES
WOS研究方向Physics
语种英语
WOS记录号WOS:000246670700009
出版者SCIENCE CHINA PRESS
公开日期2010-10-29
源URL[http://ir.imp.cas.cn/handle/113462/5973]  
专题近代物理研究所_近代物理研究所知识存储（2010之前）
通讯作者Liu Bing
作者单位1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
2.Grad Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Liu Bing,Zhang Hong,Li WenJian,et al. Pre-irradiation with low-dose C-12(6+) beam significantly enhances the efficacy of AdCMV-p53 gene therapy in human non-small lung cancer[J]. SCIENCE IN CHINA SERIES G-PHYSICS MECHANICS & ASTRONOMY,2007,50(2):221-230.
APA Liu Bing.,Zhang Hong.,Li WenJian.,Li Qiang.,Zhou GuangMing.,...&Duan Xin.(2007).Pre-irradiation with low-dose C-12(6+) beam significantly enhances the efficacy of AdCMV-p53 gene therapy in human non-small lung cancer.SCIENCE IN CHINA SERIES G-PHYSICS MECHANICS & ASTRONOMY,50(2),221-230.
MLA Liu Bing,et al."Pre-irradiation with low-dose C-12(6+) beam significantly enhances the efficacy of AdCMV-p53 gene therapy in human non-small lung cancer".SCIENCE IN CHINA SERIES G-PHYSICS MECHANICS & ASTRONOMY 50.2(2007):221-230.

入库方式： OAI收割

来源：近代物理研究所

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