Protective effects of melatonin against low- and high-LET irradiation
文献类型：期刊论文
| 作者 | Zhou, Guangming; Kawata, Tetsuya; Furusawa, Yoshiya; Aoki, Mizuho; Hirayama, Ryoichi; Ando, Koichi; Ito, Hisao |
| 刊名 | JOURNAL OF RADIATION RESEARCH
 |
| 出版日期 | 2006-06-01 |
| 卷号 | 47期号:2页码:175-181 |
| 关键词 | melatonin antioxidant mammalian cells X-rays carbon beam |
| ISSN号 | 0449-3060 |
| 英文摘要 | To investigate the protective effects of melatonin against high-LET ionizing radiation, V79 Chinese hamster cells were irradiated with 100 keV/mu m carbon beam. Parallel experiments were performed with 200 kV X-rays. To avoid the impact from extra solvents, melatonin was dissolved directly in culture medium. Cells were cultured in melatonin medium for 1 hr before irradiation. Cell inactivation was measured with conventional colony forming assay, medium containing 6-thioguanine was used for the selection of mutants at hprt locus, and the cell cycle was monitored by flow cytometry. Both carbon beam and X-rays induced cell inactivation, hprt gene mutation and cell cycle G2 block dose-dependently. But carbon beam showed stronger effects as indicated by all three endpoints and the relative biological effectiveness (RBE) was 3.5 for cell killing (at 10% survival level) and 2.9 for mutation induction (at 5 x 10(-5) mutants/ cell level). Melatonin showed protective effects against ionizing radiation in a dose-dependent manner. In terms of cell killing, melatonin only increased the survival level of those samples exposed to 8Gy or larger of X-rays or 6 Gy or larger of carbon beam. In the induction of hprt mutation and G2 block, melatonin reduced such effects induced by carbon beam but not by X-rays. The results suggest that melatonin reduces the direct interaction of particles with cells rather than an indirect interaction. Further studies are required to disclose the underlying mechanisms. |
| WOS关键词 | HUMAN SKIN FIBROBLASTS ; IONIZING-RADIATION ; MAMMALIAN-CELLS ; DAMAGE ; RATS ; PROLIFERATION ; RADIOTHERAPY ; LYMPHOCYTES |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000239836200009 |
| 出版者 | JAPAN RADIATION RESEARCH SOC |
| 公开日期 | 2010-10-29 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/6157]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） |
| 通讯作者 | Furusawa, Yoshiya |
| 作者单位 | 1.Natl Inst Radiol Sci, Heavy Ion Radiobiol Res Grp, Chiba 2638555, Japan 2.Chinese Acad Sci, Inst Modern Phys, Radiobbiol Grp, Lanzhou 730000, Peoples R China 3.Chiba Univ, Dept Radiol, Chiba 2608670, Japan |
| 推荐引用方式 GB/T 7714 | Zhou, Guangming,Kawata, Tetsuya,Furusawa, Yoshiya,et al. Protective effects of melatonin against low- and high-LET irradiation[J]. JOURNAL OF RADIATION RESEARCH,2006,47(2):175-181. |
| APA | Zhou, Guangming.,Kawata, Tetsuya.,Furusawa, Yoshiya.,Aoki, Mizuho.,Hirayama, Ryoichi.,...&Ito, Hisao.(2006).Protective effects of melatonin against low- and high-LET irradiation.JOURNAL OF RADIATION RESEARCH,47(2),175-181. |
| MLA | Zhou, Guangming,et al."Protective effects of melatonin against low- and high-LET irradiation".JOURNAL OF RADIATION RESEARCH 47.2(2006):175-181. |
入库方式： OAI收割
来源：近代物理研究所
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