Effects of ion beam irradiation on adventitious shoot regeneration from in vitro leaf explants of Saintpaulia ionahta
文献类型：期刊论文
| 作者 | Zhou, LB ; Li, WJ; Ma, S; Dong, XC; Yu, LX; Li, Q; Zhou, GM; Gao, QX
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| 刊名 | NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
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| 出版日期 | 2006-03-01 |
| 卷号 | 244期号:2页码:349-353 |
| 关键词 | mutation breeding Saintpaulia ionahta tissue culture carbon ion beam X-ray irradiation |
| ISSN号 | 0168-583X |
| DOI | 10.1016/j.nimb.2005.10.034 |
| 英文摘要 | The effects of 960 MeV carbon ion beam and 8 MeV X-ray irradiation on adventitious shoots from in vitro leaf explants of two different Saintpaulia ionahta (Mauve and Indikon) cultivars were studied with regard to tissue increase, shoots differentiation and morphology changes in the shoots. The experimental results showed that the survival fraction of shoot formation for the Mauve and Indikon irradiated with the carbon ion beam at 20 Gy were 0.715 and 0.600, respectively, while those for both the cultivars exposed to the Xray irradiation at the same dose were 1.000. Relative biological effectiveness (RBE) of Mauve with respect to X-ray was about two. Secondly, the percentage of regenerating explants with malformed shoots in all Mauve regenerating explants irradiated with carbon ion beam at 20 Gy accounted for 49.6%, while that irradiated with the same dose of X-ray irradiation was only 4.7%; as for Saintpatdia ionahta Indikon irradiated with 20 Gy carbon ion beam, the percentage was 43.3%, which was higher than that of X-ray irradiation. Last, many chlorophyll deficient and other varieties of mutants were obtained in this study. Based on the results above, it can be concluded that the effect of mutation induction by carbon ion beam irradiation on the leaf explants of Saintpaulia ionahta is better than that by X-ray irradiation; and the optimal mutagenic dose varies from 20 Gy to 25 Gy for carbon ion beam irradiation. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS关键词 | ARABIDOPSIS ; CULTURES ; GROWTH |
| WOS研究方向 | Instruments & Instrumentation ; Nuclear Science & Technology ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000235722600009 |
| 出版者 | ELSEVIER SCIENCE BV |
| 公开日期 | 2010-10-29 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/6275]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） 近代物理研究所_生物物理研究室 |
| 通讯作者 | Zhou, LB |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Dept Radiobiol, Lanzhou 730000, Peoples R China 2.Lanzhou Univ, Sch Life Sci, Inst Cell Biol, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, LB,Li, WJ,Ma, S,et al. Effects of ion beam irradiation on adventitious shoot regeneration from in vitro leaf explants of Saintpaulia ionahta[J]. NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS,2006,244(2):349-353. |
| APA | Zhou, LB.,Li, WJ.,Ma, S.,Dong, XC.,Yu, LX.,...&Gao, QX.(2006).Effects of ion beam irradiation on adventitious shoot regeneration from in vitro leaf explants of Saintpaulia ionahta.NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS,244(2),349-353. |
| MLA | Zhou, LB,et al."Effects of ion beam irradiation on adventitious shoot regeneration from in vitro leaf explants of Saintpaulia ionahta".NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS 244.2(2006):349-353. |
入库方式： OAI收割
来源：近代物理研究所
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