Potentiality of phosphorylation of BRCA1 at Ser 1524 to activate p21 in response to X-ray irradiation
文献类型：期刊论文
| 作者 | Zhao Weiping2,3,4; Li Ning2,3,4 ; Zhang Hong2,3; Wang Yanling2,3,4; Wang Xiaohu1; Hao Jifang2,3
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| 刊名 | NUCLEAR SCIENCE AND TECHNIQUES
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| 出版日期 | 2008-06-20 |
| 卷号 | 19期号:3页码:142-146 |
| 关键词 | BRCA1 phosphorylation cell cycle apoptosis |
| ISSN号 | 1001-8042 |
| 英文摘要 | The breast and ovarian cancer susceptibility gene BRCA1 encodes a nuclear phosphoprotein, which functions as a tumor suppressor gene. Many studies suggested that multiple functions of BRCA1 may contribute to its tumor suppressor activity, including roles in cell cycle checkpoints, apoptosis and transcription. It is postulated that phosphorylation of BRCA1 is an important means by which its cellular functions are regulated. In this study, we employed phospho-Ser-specific antibody recognizing Ser-1524 to study BRCA1 phosphorylation under conditions of DNA damage and the effects of phosphorylation on BRCA1 functions. The results showed that 10 Gy X-ray treatment significantly induced phosphorylation of Ser-1524 but not total BRCA1 protein levels. The expression both of p53 and p21 increased after irradiation, but ionizing radiation (IR) -induced activation of p21 was prior to that of p53. The percentages of G0/G1 phase remarkably increased after IR. In addition, no detectable levels of 89 kDa fragment of PARP, a marker of apoptotic cells, were observed. Data implied that IR-induced phosphorylation of BRCA1 at Ser-1524 might activatep21 protein, by which BRCA1 regulated cell cycle, but play no role in apoptosis. |
| WOS关键词 | DNA-DAMAGE RESPONSE ; STRAND BREAK REPAIR ; CELL-CYCLE ; NUCLEAR PHOSPHOPROTEIN ; TUMOR-SUPPRESSOR ; ARREST ; P21(WAF1/CIP1) ; CANCER ; P53 ; ATM |
| 资助项目 | National Natural Science Foundation of China[10675151] ; Key Scientific Technology Research Projects of Gansu Province[O702NKDA045] ; Scientific Technology Research Project of Lanzhou-Chinese Academy of Sciences[07-2-07] ; Western Talent Program of Chinese Academy of Sciences[0760160NBO] ; Western Talent Program of Chinese Academy of Sciences[0860260XBO] |
| WOS研究方向 | Nuclear Science & Technology ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000258910300004 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | National Natural Science Foundation of China ; Key Scientific Technology Research Projects of Gansu Province ; Scientific Technology Research Project of Lanzhou-Chinese Academy of Sciences ; Western Talent Program of Chinese Academy of Sciences |
| 公开日期 | 2010-10-29 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/6327]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） |
| 通讯作者 | Zhang Hong |
| 作者单位 | 1.Gansu Tumor Hosp, Dept Radiotherapy, Lanzhou 730050, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao Weiping,Li Ning,Zhang Hong,et al. Potentiality of phosphorylation of BRCA1 at Ser 1524 to activate p21 in response to X-ray irradiation[J]. NUCLEAR SCIENCE AND TECHNIQUES,2008,19(3):142-146. |
| APA | Zhao Weiping,Li Ning,Zhang Hong,Wang Yanling,Wang Xiaohu,&Hao Jifang.(2008).Potentiality of phosphorylation of BRCA1 at Ser 1524 to activate p21 in response to X-ray irradiation.NUCLEAR SCIENCE AND TECHNIQUES,19(3),142-146. |
| MLA | Zhao Weiping,et al."Potentiality of phosphorylation of BRCA1 at Ser 1524 to activate p21 in response to X-ray irradiation".NUCLEAR SCIENCE AND TECHNIQUES 19.3(2008):142-146. |
入库方式： OAI收割
来源：近代物理研究所
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