Radio-resistance induced by nitric oxide to heavy ion irradiation in A172 human glioma cells
文献类型：期刊论文
| 作者 | Zhou Qingming2,3; Zhang Hong2 ; Zhang Xingxia1
|
| 刊名 | NUCLEAR SCIENCE AND TECHNIQUES
 |
| 出版日期 | 2007-10-20 |
| 卷号 | 18期号:5页码:282-286 |
| 关键词 | nitric oxide C-12(6+) heavy ion A172 human glioma cells radio-resistance |
| ISSN号 | 1001-8042 |
| 英文摘要 | To investigate effects of nitric oxide on cellular radio-sensitivity, three human glioma cell lines, i.e. A172, A172 transfected green fluorescence protein (EGFP) gene (EA172) and A172 transfected inducible nitric oxide synthesis (iNOS) gene (iA72), were irradiated by C-12(6+) ions to 0, 1 or My. Productions of nitric oxide and glutathione (GSH) in A172, EA172 and iA172 were determined by chemical methods, cell cycle was analyzed by flow cytometry at the 24th hour after irradiation, and survival fraction of the cells was measured by colorimetric MTT assay at the 5th day after irradiation. The results showed that the concentrations of nitric oxide and GSH in iA172 were significantly higher than in A172 and EA172; the G(2)/M stage arrest induced by the C-12(6+) ion irradiation was observed in A172 and EA172 but not in iA172 at the 24th hour after exposure; and the survival fraction of iA172 was higher than that of EA172 and iA172. Data suggest that the radio-sensitivity of the A172 was reduced after the iNOS gene transfection. The increase of GSH production and the change of cellular signals such as the cell cycle control induced by nitric oxide may be involved in this radio-resistance. |
| WOS关键词 | RADIORESISTANCE ; INDUCTION ; P53 |
| WOS研究方向 | Nuclear Science & Technology ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000255053100006 |
| 出版者 | ELSEVIER SCIENCE BV |
| 公开日期 | 2010-10-29 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/6335]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） |
| 通讯作者 | Zhang Hong |
| 作者单位 | 1.Lanzhou Univ, Hosp 1st, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou Qingming,Zhang Hong,Zhang Xingxia. Radio-resistance induced by nitric oxide to heavy ion irradiation in A172 human glioma cells[J]. NUCLEAR SCIENCE AND TECHNIQUES,2007,18(5):282-286. |
| APA | Zhou Qingming,Zhang Hong,&Zhang Xingxia.(2007).Radio-resistance induced by nitric oxide to heavy ion irradiation in A172 human glioma cells.NUCLEAR SCIENCE AND TECHNIQUES,18(5),282-286. |
| MLA | Zhou Qingming,et al."Radio-resistance induced by nitric oxide to heavy ion irradiation in A172 human glioma cells".NUCLEAR SCIENCE AND TECHNIQUES 18.5(2007):282-286. |
入库方式： OAI收割
来源：近代物理研究所
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