Study on DNA Damage Induced by Neon Beam Irradiation in Saccharomyces Cerevisiae
文献类型：期刊论文
| 作者 | Dai Zhongying1,2 ; Lu Dong1,2; Li Wenjian1; Wu Xin1,2; Wang Jufang1; Ma Shuang1; Liu Qingfang1,2; He Jinyu1,2; Jing Xigang1; Ding Nan1,2
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| 刊名 | PLASMA SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2010-12-01 |
| 卷号 | 12期号:6页码:753-756 |
| 关键词 | neon ion beam irradiation yeast DSBs mutation |
| ISSN号 | 1009-0630 |
| 英文摘要 | Yeast strain Saccharornyces cerevisiae was irradiated with different doses of 85 MeV/u (20)Ne(10+) to investigate DNA damage induced by heavy ion beam in eukaryotic microorganism. The survival rate, DNA double strand breaks (DSBs) and DNA polymorphic were tested after irradiation. The results showed that there were substantial differences in DNA between the control and irradiated samples. At the dose of 40 Cy, the yeast cell survival rate approached 50%, DNA double-strand breaks were barely detectable, and significant DNA polymorphism was observed. The alcohol dehydrogenase II gene was amplified and sequenced. It was observed that base changes in the mutant were mainly transversions of T-->G and T-->C. It can be concluded that heavy ion beam irradiation can lead to change in single gene and may be an effective way to induce mutation. |
| WOS关键词 | RAPD ASSAY ; MUTATIONS ; IONS ; DIVERSITY ; PFGE |
| 资助项目 | Natural Science Foundation of Gansu Province, Department of Science And Technology, Gansu Province, China[096RJZA126] |
| WOS研究方向 | Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000286172000021 |
| 出版者 | IOP PUBLISHING LTD |
| 资助机构 | Natural Science Foundation of Gansu Province, Department of Science And Technology, Gansu Province, China |
| 公开日期 | 2011-04-20 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/7777]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） 近代物理研究所_生物物理研究室 |
| 通讯作者 | Lu Dong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Dept Biophys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100040, Peoples R China 3.Gansu Prov Acad Sci, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai Zhongying,Lu Dong,Li Wenjian,et al. Study on DNA Damage Induced by Neon Beam Irradiation in Saccharomyces Cerevisiae[J]. PLASMA SCIENCE & TECHNOLOGY,2010,12(6):753-756. |
| APA | Dai Zhongying.,Lu Dong.,Li Wenjian.,Wu Xin.,Wang Jufang.,...&Zhou Jianping.(2010).Study on DNA Damage Induced by Neon Beam Irradiation in Saccharomyces Cerevisiae.PLASMA SCIENCE & TECHNOLOGY,12(6),753-756. |
| MLA | Dai Zhongying,et al."Study on DNA Damage Induced by Neon Beam Irradiation in Saccharomyces Cerevisiae".PLASMA SCIENCE & TECHNOLOGY 12.6(2010):753-756. |
入库方式： OAI收割
来源：近代物理研究所
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