中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 近代物理研究所
  3. 中国科学院近代物理研究所
  4. 近代物理研究所知识存储（2010之前）
BRCA1 and its phosphorylation involved in caffeine-inhibitable event upstream of G2 checkpoint

文献类型：期刊论文

作者Zhang Hong1,3,4; Wang YanLing1,3,4; Li Ning1,2,3,4; Hao JiFang1,3,4
刊名SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
出版日期2010-07-01
卷号53期号:7页码:1281-1285
关键词BRCA1 phosphorylation ionizing irradiation caffeine G2 checkpoint
ISSN号1674-7348
DOI10.1007/s11433-010-4022-1
英文摘要Caffeine, which specifically inhibits ATM/ATR kinases, efficiently abrogates the ionizing radiation (IR)-induced G2 arrest and increases the sensitivity of various tumor cells to IR. Mechanisms for the effect of caffeine remain to be elucidated. As a target of ATM/ATR kinases, BRCA1 becomes activated and phosphorylated in response to IR. Thus, in this work, we investigated the possible role of BRCA1 in the effect of caffeine on G2 checkpoint and observed how BRCA1 phosphorylation was regulated in this process. For these purposes, the BRCA1 protein level and the phosphorylation states were analyzed by Western blotting by using an antibody against BRCA1 and phospho-specific antibodies against Ser-1423 and Ser-1524 residues in cells exposed to a combination of IR and caffeine. The results showed that caffeine down-regulated IR-induced BRCA1 expression and specifically abolished BRCA1 phosphorylation of Ser-1524, which was followed by an override of G2 arrest by caffeine. In addition, the ability of BRCA1 to transactivate p21 may be required for MCF-7 but not necessary for Hela response to caffeine. These data suggest that BRCA1 may be a potential target of caffeine. BRCA1 and its phosphorylation are most likely to be involved in the caffeine-inhibitable event upstream of G2 arrest.
WOS关键词DNA-DAMAGE CHECKPOINT ; DOUBLE-STRAND BREAKS ; CELL-CYCLE ; IONIZING IRRADIATION ; ATM ; KINASE ; DISTINCT ; ARREST ; TRANSACTIVATION ; ACTIVATION
资助项目National Basic Research Program of China[2010CB834202] ; National Natural Science Foundation of China[10835011] ; National Natural Science Foundation of China[10805064] ; Scientific Technology Research Projects of Gansu Province[0806RJYA020] ; Scientific Technology Research Projects of Gansu Province[099RJYA015] ; Western Talent Program of Chinese Academy of Sciences[0860260XBO]
WOS研究方向Physics
语种英语
WOS记录号WOS:000280210700022
出版者SCIENCE PRESS
资助机构National Basic Research Program of China ; National Natural Science Foundation of China ; Scientific Technology Research Projects of Gansu Province ; Western Talent Program of Chinese Academy of Sciences
公开日期2011-04-20
源URL[http://ir.imp.cas.cn/handle/113462/8023]  
专题近代物理研究所_近代物理研究所知识存储（2010之前）
通讯作者Zhang Hong
作者单位1.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China
4.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714		 Zhang Hong,Wang YanLing,Li Ning,et al. BRCA1 and its phosphorylation involved in caffeine-inhibitable event upstream of G2 checkpoint[J]. SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2010,53(7):1281-1285.
APA Zhang Hong,Wang YanLing,Li Ning,&Hao JiFang.(2010).BRCA1 and its phosphorylation involved in caffeine-inhibitable event upstream of G2 checkpoint.SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,53(7),1281-1285.
MLA Zhang Hong,et al."BRCA1 and its phosphorylation involved in caffeine-inhibitable event upstream of G2 checkpoint".SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY 53.7(2010):1281-1285.

入库方式： OAI收割

来源：近代物理研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。