Biphasic regulation of H2O2 on angiogenesis implicated NADPH oxidase
文献类型：期刊论文
| 作者 | Mu, Ping2; Liu, Qing3; Zheng, Rongliang1,2 |
| 刊名 | CELL BIOLOGY INTERNATIONAL
 |
| 出版日期 | 2010-10-01 |
| 卷号 | 34期号:10页码:1013-1020 |
| 关键词 | angiogenesis hydrogen peroxide NADPH oxidase |
| ISSN号 | 1065-6995 |
| DOI | 10.1042/CBI20090092 |
| 英文摘要 | ROS (reactive oxygen species) take an important signalling role in angiogenesis. Although there are several ways to produce ROS in cells, multicomponent non-phagocytic NADPH oxidase is an important source of ROS that contribute to angiogenesis. In the present work, we examined the effects of H2O2 on angiogenesis including proliferation and migration in HUVECs (human umbilical vein endothelial cells), new vessel formation in chicken embryo CAM (chorioallantoic membrane) and endothelial cell apoptosis, which is closely related to anti-angiogenesis. Our results showed that H2O2 dose-dependently increased the generation of O-2(-) (superoxide anion) in HUVECs, which was suppressed by DPI (diphenylene iodonium) and APO (apocynin), two inhibitors of NADPH oxidase. H2O2 at low concentrations (10 mu M) stimulated cell proliferation and migration, but at higher concentrations, inhibited both. Similarly, H2O2 at 4 nmol/cm(2) strongly induced new vessel formation in CAM, while it suppressed at high concentrations (higher than 4 nmol/cm(2)). Also, H2O2 (200 similar to 500 mu M) could stimulate apoptosis in HUVECs. All the effects of H2O2 on angiogenesis could be suppressed by NADPH oxidase inhibitors, which suggests that NADPH oxidase acts downstream of H2O2 to produce O-2(-) and then to regulate angiogenesis. In summary, our results suggest that H2O2 as well as O-2(-) mediated by NADPH oxidase have biphasic effects on angiogenesis in vitro and in vivo. |
| WOS关键词 | VASCULAR NAD(P)H OXIDASES ; HUMAN ENDOTHELIAL-CELLS ; HYDROGEN-PEROXIDE ; IN-VITRO ; ANGIOTENSIN ; EXPRESSION ; MEDIATORS ; MECHANISM ; VIVO |
| 资助项目 | E-Institutes of Shanghai Municipal Education Commission[E-04010] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000283533400007 |
| 出版者 | PORTLAND PRESS LTD |
| 资助机构 | E-Institutes of Shanghai Municipal Education Commission |
| 公开日期 | 2011-05-16 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/8277]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） |
| 通讯作者 | Zheng, Rongliang |
| 作者单位 | 1.Shanghai Univ Tradit Chinese Med, E Inst Shanghai Univ, Div Nitr Oxide & Inflammatory Med, Shanghai 201203, Peoples R China 2.Lanzhou Univ, Inst Biophys, Sch Life Sci, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Dept Radiol, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mu, Ping,Liu, Qing,Zheng, Rongliang. Biphasic regulation of H2O2 on angiogenesis implicated NADPH oxidase[J]. CELL BIOLOGY INTERNATIONAL,2010,34(10):1013-1020. |
| APA | Mu, Ping,Liu, Qing,&Zheng, Rongliang.(2010).Biphasic regulation of H2O2 on angiogenesis implicated NADPH oxidase.CELL BIOLOGY INTERNATIONAL,34(10),1013-1020. |
| MLA | Mu, Ping,et al."Biphasic regulation of H2O2 on angiogenesis implicated NADPH oxidase".CELL BIOLOGY INTERNATIONAL 34.10(2010):1013-1020. |
入库方式： OAI收割
来源：近代物理研究所
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