Loss of p15/Ink4b accompanies tumorigenesis triggered by complex DNA double-strand breaks
文献类型：期刊论文
| 作者 | Habib, Amyn A.5,6; Xie, Xian-Jin1; Nirodi, Chaitanya S.; Saha, Debabrata; Story, Michael D.; Balajee, Adayabalam S.3; Bachoo, Robert M.5; Boothman, David A.1,7; Burma, Sandeep2; Camacho, Cristel V. |
| 刊名 | CARCINOGENESIS
 |
| 出版日期 | 2010-10-01 |
| 卷号 | 31期号:10页码:1889-1896 |
| ISSN号 | 0143-3334 |
| DOI | 10.1093/carcin/bgq153 |
| 英文摘要 | DNA double-strand breaks (DSBs) are the most deleterious lesion inflicted by ionizing radiation. Although DSBs are potentially carcinogenic, it is not clear whether complex DSBs that are refractory to repair are more potently tumorigenic compared with simple breaks that can be rapidly repaired, correctly or incorrectly, by mammalian cells. We previously demonstrated that complex DSBs induced by high-linear energy transfer (LET) Fe ions are repaired slowly and incompletely, whereas those induced by low-LET gamma rays are repaired efficiently by mammalian cells. To determine whether Fe-induced DSBs are more potently tumorigenic than gamma ray-induced breaks, we irradiated 'sensitized' murine astrocytes that were deficient in Ink4a and Arf tumor suppressors and injected the surviving cells subcutaneously into nude mice. Using this model system, we find that Fe ions are potently tumorigenic, generating tumors with significantly higher frequency and shorter latency compared with tumors generated by gamma rays. Tumor formation by Fe-irradiated cells is accompanied by rampant genomic instability and multiple genomic changes, the most interesting of which is loss of the p15/Ink4b tumor suppressor due to deletion of a chromosomal region harboring the CDKN2A and CDKN2B loci. The additional loss of p15/Ink4b in tumors derived from cells that are already deficient in p16/Ink4a bolsters the hypothesis that p15 plays an important role in tumor suppression, especially in the absence of p16. Indeed, we find that reexpression of p15 in tumor-derived cells significantly attenuates the tumorigenic potential of these cells, indicating that p15 loss may be a critical event in tumorigenesis triggered by complex DSBs. |
| WOS关键词 | EPITHELIAL-CELLS ; MALIGNANT-TRANSFORMATION ; SPACE EXPLORATION ; TUMOR-SUPPRESSOR ; RADIATION ; CANCER ; LOCUS ; CARCINOGENESIS ; PARTICLES ; TELOMERES |
| 资助项目 | National Aeronautics and Space Administration[NNA05CS97G] ; National Aeronautics and Space Administration[NNX10AE08G] ; Cancer Prevention and Research Institute of Texas[RP100644] ; Goldhirsh Foundation ; National Institutes of Health[CA122972] ; National Institutes of Health[CA129364] ; National Institutes of Health[NS062080] ; National Cancer Institute[T32CA124334] ; Department of Energy[DE-FG02-05ER64055] ; National Aeronautics and Space Administration Specialized Center of Research[NNJ05HD36G] ; Flight Attendant Medical Research Institute[CSCN 050] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000282750100024 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Aeronautics and Space Administration ; Cancer Prevention and Research Institute of Texas ; Goldhirsh Foundation ; National Institutes of Health ; National Cancer Institute ; Department of Energy ; National Aeronautics and Space Administration Specialized Center of Research ; Flight Attendant Medical Research Institute |
| 公开日期 | 2011-05-16 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/8279]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） 近代物理研究所_空间辐射生物研究室 |
| 通讯作者 | Burma, Sandeep |
| 作者单位 | 1.Univ Texas SW Med Ctr Dallas, Simmons Comprehens Canc Ctr, Dallas, TX 75390 USA 2.Univ Texas SW Med Ctr Dallas, Dept Radiat Oncol, Div Mol Radiat Biol, Dallas, TX 75390 USA 3.Columbia Univ, Ctr Radiol Res, Dept Radiat Oncol, New York, NY 10032 USA 4.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China 5.Univ Texas SW Med Ctr Dallas, Dept Neurol, Dallas, TX 75390 USA 6.N Texas VA Med Ctr, Dept Neurol, Dallas, TX 75216 USA 7.Univ Texas SW Med Ctr Dallas, Dept Pharmacol, Dallas, TX 75390 USA |
| 推荐引用方式 GB/T 7714 | Habib, Amyn A.,Xie, Xian-Jin,Nirodi, Chaitanya S.,et al. Loss of p15/Ink4b accompanies tumorigenesis triggered by complex DNA double-strand breaks[J]. CARCINOGENESIS,2010,31(10):1889-1896. |
| APA | Habib, Amyn A..,Xie, Xian-Jin.,Nirodi, Chaitanya S..,Saha, Debabrata.,Story, Michael D..,...&Hu, Burong.(2010).Loss of p15/Ink4b accompanies tumorigenesis triggered by complex DNA double-strand breaks.CARCINOGENESIS,31(10),1889-1896. |
| MLA | Habib, Amyn A.,et al."Loss of p15/Ink4b accompanies tumorigenesis triggered by complex DNA double-strand breaks".CARCINOGENESIS 31.10(2010):1889-1896. |
入库方式： OAI收割
来源：近代物理研究所
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