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Live cell imaging combined with high-energy single-ion microbeam

文献类型：期刊论文

作者Guo, Jinlong1,2; Liu, Wenjing1; Du, Guanghua1; Wu, Ruqun1; Chen, Hao1,3; Guo, Na1; Wei, Junzhe1
刊名REVIEW OF SCIENTIFIC INSTRUMENTS
出版日期2016-03-01
卷号87页码:5
ISSN号0034-6748
DOI10.1063/1.4943257
英文摘要DNA strand breaks can lead to cell carcinogenesis or cell death if not repaired rapidly and efficiently. An online live cell imaging system was established at the high energy microbeam facility at the Institute of Modern Physics to study early and fast cellular response to DNA damage after high linear energy transfer ion radiation. The HT1080 cells expressing XRCC1-RFP were irradiated with single high energy nickel ions, and time-lapse images of the irradiated cells were obtained online. The live cell imaging analysis shows that strand-break repair protein XRCC1 was recruited to the ion hit position within 20 s in the cells and formed bright foci in the cell nucleus. The fast recruitment of XRCC1 at the ion hits reached a maximum at about 200 s post-irradiation and then was followed by a slower release into the nucleoplasm. The measured dual-exponential kinetics of XRCC1 protein are consistent with the proposed consecutive reaction model, and the measurements obtained that the reaction rate constant of the XRCC1 recruitment to DNA strand break is 1.2 x 10(-3) s(-1) and the reaction rate constant of the XRCC1 release from the break-XRCC1 complex is 1.2 x 10(-2) s(-1). (C) 2016 AIP Publishing LLC.
WOS关键词STRAND BREAK REPAIR ; BASE EXCISION-REPAIR ; DNA ; RADIATION ; XRCC1 ; THERAPY ; PATHWAY
资助项目100 Talents Program of Chinese Academy of Sciences (CAS)[Y150310BRO] ; National Natural Science Foundation of China (NNSFC)[31200630] ; Ministry of Science and Technology[2012YQ03014204] ; project Development of the Key Equipment for Research of CAS[O713040YZ0]
WOS研究方向Instruments & Instrumentation ; Physics
语种英语
WOS记录号WOS:000373713300041
出版者AMER INST PHYSICS
资助机构100 Talents Program of Chinese Academy of Sciences (CAS) ; National Natural Science Foundation of China (NNSFC) ; Ministry of Science and Technology ; project Development of the Key Equipment for Research of CAS
源URL[http://119.78.100.186/handle/113462/42192]  
专题近代物理研究所_实验物理中心
通讯作者Du, Guanghua
作者单位1.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China
2.Northwest Normal Univ, Lanzhou, Peoples R China
3.Lanzhou Univ, Inst Nucl Sci & Technol, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714		 Guo, Jinlong,Liu, Wenjing,Du, Guanghua,et al. Live cell imaging combined with high-energy single-ion microbeam[J]. REVIEW OF SCIENTIFIC INSTRUMENTS,2016,87:5.
APA Guo, Jinlong.,Liu, Wenjing.,Du, Guanghua.,Wu, Ruqun.,Chen, Hao.,...&Wei, Junzhe.(2016).Live cell imaging combined with high-energy single-ion microbeam.REVIEW OF SCIENTIFIC INSTRUMENTS,87,5.
MLA Guo, Jinlong,et al."Live cell imaging combined with high-energy single-ion microbeam".REVIEW OF SCIENTIFIC INSTRUMENTS 87(2016):5.

入库方式： OAI收割

来源：近代物理研究所

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