Influence of the environment and phototoxicity of the live cell imaging system at IMP microbeam facility
文献类型：会议论文
| 作者 | Liu, Wenjing1; Wei, Junzhe1,2; Wu, Ruqun1,2; Guo, Jinlong1,3 ; Du, Guanghua1; Li, Xiaoyue1,2; Zhao, Jing1,2; Li, Yaning1,2; Chen, Hao4
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| 出版日期 | 2017-08 |
| 关键词 | Live cell imaging DNA damage and repair Phototoxicity Environment control Microbeam |
| 卷号 | 404 |
| DOI | 10.1016/j.nimb.2017.01.076 |
| 页码 | 125-130 |
| 英文摘要 | To investigate the spatiotemporal dynamics of DNA damage and repair after the ion irradiation, an online live cell imaging system has been established based on the microbeam facility at Institute of Modern Physics (IMP). The system could provide a sterile and physiological environment by making use of heating plate and live cell imaging solution. The phototoxicity was investigated through the evaluation of DNA repair protein XRCC1 foci formed in HT1080-RFP cells during the imaging exposure. The intensity of the foci induced by phototoxicity was much lower compared with that of the foci induced by heavy ion hits. The results showed that although spontaneous foci were formed due to RFP exposure during live cell imaging, they had little impact on the analysis of the recruitment kinetics of XRCC1 in the foci induced by the ion irradiation. (C) 2017 Elsevier B.V. All rights reserved. |
| 会议录 | NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
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| 会议录出版者 | ELSEVIER SCIENCE BV |
| 会议录出版地 | PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| 语种 | 英语 |
| 资助项目 | Ministry of Science and Technology[2012YQ03014204] |
| WOS研究方向 | Instruments & Instrumentation ; Nuclear Science & Technology ; Physics |
| WOS记录号 | WOS:000404709900024 |
| 源URL | [http://119.78.100.186/handle/113462/58157]  |
| 专题 | 近代物理研究所_实验物理中心 |
| 通讯作者 | Du, Guanghua |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Northwest Normal Univ, Lanzhou, Peoples R China 4.Univ Lanzhou, Lanzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Wenjing,Wei, Junzhe,Wu, Ruqun,et al. Influence of the environment and phototoxicity of the live cell imaging system at IMP microbeam facility[C]. 见:. |
入库方式： OAI收割
来源：近代物理研究所
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