The antioxidant mechanism of nitroxide TEMPO: scavenging with glutathionyl radicals
文献类型：期刊论文
| 作者 | Wang, Shuai1,2; Li, Qiang4 ; Wu, Qingfeng4; Li, Feifei3,4; Cao, Linying1,2,3; Yu, Hui1,2,3; Guo, Yong1,2
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| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2015 |
| 卷号 | 5页码:63655-63661 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c5ra06129f |
| 英文摘要 | A rhodamine-nitroxide probe (R-NOc), combining rhodamine fluorophore with a 2,2,6,6-tetramethylpiperidinyl-1-oxy (TEMPO) receptor unit was introduced to probe glutathionyl radicals (GSc) with high sensitivity and selectivity. The R-NOc probe could effectively scavenge GSc radicals with fluorescence enhancement since the nitroxide group restored the fluorescence properties. In this work, horseradish peroxidase (HRP)-catalyzed and metal-catalyzed oxidation systems were selected as the model of simulating the generation of GSc, and we found that the metal-catalyzed system had the same experimental results with the HRP-catalyzed system, which provided a new approach to demonstrate the strong oxidant ability of the hydroxyl radical (cOH) to initiate toxic GSc. Furthermore, we confirmed that the production of GSc abided by a radical-initiated peroxidation mechanism of GSH with the mass spectrometry (MS) analysis and fluorescence spectroscopy. By using combined high-performance liquid chromatography (HPLC) detection and MS analysis, we also demonstrated that the R-NOc was converted into fluorescent secondary amine derivative (R-NH). The application of the probe in biological system was explored to monitor GSc in HL-60 cells and secondary amine fluorescence was observed upon stimulation by hydrogen peroxide and phenol. Development of fluorescence was prevented via preincubation with the thiol-blocking reagent N-ethylmaleimide (NEM). |
| WOS关键词 | THIYL RADICALS ; FLUORESCENCE PROBE ; REDOX ENVIRONMENT ; REACTIVE OXYGEN ; HUMAN-DISEASE ; NITRIC-OXIDE ; DAMAGE ; KINETICS ; CELLS ; MYELOPEROXIDASE |
| 资助项目 | Around five top priorities program of "One-Three-Five" Strategic Planning of Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences ; National Science & Technology Major Project of China[2011ZX05011] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000358734800061 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | Around five top priorities program of "One-Three-Five" Strategic Planning of Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences ; National Science & Technology Major Project of China |
| 源URL | [http://ir.impcas.ac.cn/handle/113462/18575]  |
| 专题 | 近代物理研究所_医学物理研究室 近代物理研究所_生物物理研究室 |
| 通讯作者 | Guo, Yong |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Shuai,Li, Qiang,Wu, Qingfeng,et al. The antioxidant mechanism of nitroxide TEMPO: scavenging with glutathionyl radicals[J]. RSC ADVANCES,2015,5:63655-63661. |
| APA | Wang, Shuai.,Li, Qiang.,Wu, Qingfeng.,Li, Feifei.,Cao, Linying.,...&Guo, Yong.(2015).The antioxidant mechanism of nitroxide TEMPO: scavenging with glutathionyl radicals.RSC ADVANCES,5,63655-63661. |
| MLA | Wang, Shuai,et al."The antioxidant mechanism of nitroxide TEMPO: scavenging with glutathionyl radicals".RSC ADVANCES 5(2015):63655-63661. |
入库方式： OAI收割
来源：近代物理研究所
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