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Fluorescence and HPLC Detection of Hydroxyl Radical by a Rhodamine-Nitroxide Probe and its Application in Cell Imaging

文献类型：期刊论文

作者Guo, Y (reprint author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China.; Shao, SJ5; Cao, LY5; Wu, QF5; Li, Q5; Guo, Y5
刊名JOURNAL OF FLUORESCENCE
出版日期2014
卷号24页码:313-318
关键词Spin-trapping Evidence Living Cells Oxidative Stress Generation Assay Peroxynitrite Mitochondria Antioxidant Damage
ISSN号1053-0509
DOI10.1007/s10895-013-1329-0
英文摘要A rhodamine nitroxide probe was designed to detect the hydroxyl radical (center dot OH), which presented high selectivity for center dot OH over other reactive oxygen species (ROS) and linear fluorescence response to center dot OH produced by Fenton reaction. The product was detected by HPLC-MS, indicating that the main product of the reaction was O-methylhydroxylamine and the product peak areas measured by HPLC-UV/vis and HPLC-FLD both enhanced proportionally with the increase of center dot OH concentration. The application of the probe in biological system was explored to trace the production of center dot OH in cells under oxidative stress condition induced by rotenone which can inhibit the mitochondria respiratory chain complex I and we found that appropriate rotenone may induce the normal human liver cells (L02) and human hepatoma cells (HepG2) to produce center dot OH at different degrees.
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WOS关键词SPIN-TRAPPING EVIDENCE ; LIVING CELLS ; OXIDATIVE STRESS ; GENERATION ; ASSAY ; PEROXYNITRITE ; MITOCHONDRIA ; ANTIOXIDANT ; DAMAGE
资助项目Natural Science Foundation of Gansu province[096RJ2A033]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000334183100006
出版者SPRINGER/PLENUM PUBLISHERS
资助机构Ministry of Science and Technology [2006BAI06A18-16, 2012YQ040140, 2008BAK03A02] ; Ministry of Science and Technology [2006BAI06A18-16, 2012YQ040140, 2008BAK03A02] ; Natural Science Foundation of China [81171206] ; Natural Science Foundation of China [81171206]
源URL[http://ir.impcas.ac.cn/handle/113462/18581]  
专题近代物理研究所_医学物理研究室
近代物理研究所_生物物理研究室
通讯作者Guo, Y (reprint author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China.
作者单位1.Gong, Li
2.Li, Qiang] Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Gansu, Peoples R China
3.[Liu, Xiongxiong
4.Ye, Fei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.[Liu, Xiongxiong
6.Sun, Chao
7.Jin, Xiaodong
8.Li, Qiang] Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China
9.[Liu, Xiongxiong
10.Gong, Li
推荐引用方式
GB/T 7714		 Guo, Y ,Shao, SJ,Cao, LY,et al. Fluorescence and HPLC Detection of Hydroxyl Radical by a Rhodamine-Nitroxide Probe and its Application in Cell Imaging[J]. JOURNAL OF FLUORESCENCE,2014,24:313-318.
APA Guo, Y ,Shao, SJ,Cao, LY,Wu, QF,Li, Q,&Guo, Y.(2014).Fluorescence and HPLC Detection of Hydroxyl Radical by a Rhodamine-Nitroxide Probe and its Application in Cell Imaging.JOURNAL OF FLUORESCENCE,24,313-318.
MLA Guo, Y ,et al."Fluorescence and HPLC Detection of Hydroxyl Radical by a Rhodamine-Nitroxide Probe and its Application in Cell Imaging".JOURNAL OF FLUORESCENCE 24(2014):313-318.

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来源：近代物理研究所

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