Radiosensitization by Inhibiting Survivin in Human Hepatoma HepG2 Cells to High-LET Radiation
文献类型：期刊论文
| 作者 | Hao, Jifang3,4; Jin, Xiaodong3,4 ; Gong, Li3,4; Dai, Zhongying2,3,4; Furusawa, Yoshiya1; Matsumoto, Yoshitaka1; Li, Ping2,3,4; Wu, Qingfeng2,3,4; Li, Qiang3,4
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| 刊名 | JOURNAL OF RADIATION RESEARCH
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| 出版日期 | 2011-05-01 |
| 卷号 | 52页码:335-341 |
| 关键词 | High-LET radiation Apoptosis Survivin siRNA |
| ISSN号 | 0449-3060 |
| DOI | 10.1269/jrr.10134 |
| 英文摘要 | In this study, whether survivin plays a direct role in mediating high-LET radiation resistance in human hepatoma cells was investigated. Small interfering RNA (siRNA) targeting survivin mRNA was designed and transfected into human hepatoma HepG2 cells. Real-time PCR and western blotting analyses revealed that survivin expression in HepG2 cells decreased at both transcriptional and post-transcriptional levels after treatment with survivin-specific siRNA. Caspase-3 activity was determined with a microplate reader assay as well. Following exposure to high-LET carbon ions, a reduced clonogenic survival effect, increased apoptotic rates and caspase-3 activity were observed in the cells treated with the siRNA compared to those untreated with the siRNA. The cells with transfection of the survivin-specific siRNA also increased the level of G(2)/M arrest. These results suggest that survivin definitely plays a role in mediating the resistance of HepG2 cells to high-LET radiation and depressing survivin expression might be useful to improve the therapeutic efficacy of heavy ions for radioresistant solid tumors. |
| WOS关键词 | THERAPEUTIC TARGET ; RADIORESISTANCE FACTOR ; PANCREATIC-CANCER ; APOPTOSIS ; SIRNA ; EXPRESSION |
| 资助项目 | National Basic Research Program of China (973 Program)[2010CB834203] ; Chinese Academy of Sciences[0962030XBO] ; National Natural Science Foundation of China[10905080] ; National Natural Science Foundation of China[11075191] ; Science and Technology Development Project of Lanzhou City[2008-sr-10] ; Science and Technology Development Project of Lanzhou City[2009-2-12] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000291525000011 |
| 出版者 | JAPAN RADIATION RESEARCH SOC |
| 资助机构 | National Basic Research Program of China (973 Program) ; Chinese Academy of Sciences ; National Natural Science Foundation of China ; Science and Technology Development Project of Lanzhou City |
| 源URL | [http://ir.impcas.ac.cn/handle/113462/18589]  |
| 专题 | 近代物理研究所_医学物理研究室 近代物理研究所_生物物理研究室 |
| 通讯作者 | Li, Qiang |
| 作者单位 | 1.Natl Inst Radiol Sci, Res Ctr Heavy Ion Therapy, Inage Ku, Chiba 260, Japan 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 4.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Jifang,Jin, Xiaodong,Gong, Li,et al. Radiosensitization by Inhibiting Survivin in Human Hepatoma HepG2 Cells to High-LET Radiation[J]. JOURNAL OF RADIATION RESEARCH,2011,52:335-341. |
| APA | Hao, Jifang.,Jin, Xiaodong.,Gong, Li.,Dai, Zhongying.,Furusawa, Yoshiya.,...&Li, Qiang.(2011).Radiosensitization by Inhibiting Survivin in Human Hepatoma HepG2 Cells to High-LET Radiation.JOURNAL OF RADIATION RESEARCH,52,335-341. |
| MLA | Hao, Jifang,et al."Radiosensitization by Inhibiting Survivin in Human Hepatoma HepG2 Cells to High-LET Radiation".JOURNAL OF RADIATION RESEARCH 52(2011):335-341. |
入库方式： OAI收割
来源：近代物理研究所
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