Relationship between cellular radio-sensitivity and naked DNA damage in mammalian cells exposed to heavy ions
文献类型：期刊论文
| 作者 | Li Qiang ; Wang Jufang; Li Wenjian; Zhou Guangming; Wang Zhuanzi; Dang Bingrong
|
| 刊名 | NUCLEAR SCIENCE AND TECHNIQUES
 |
| 出版日期 | 2009-12-20 |
| 卷号 | 20页码:349-353 |
| 关键词 | Heavy ion Mammalian cell Radio-sensitivity DNA damage |
| ISSN号 | 1001-8042 |
| 英文摘要 | The relationship between deoxyribonucleic acid (DNA) damage and the cell death induced by (12)C ions irradiation was examined in four kinds of cells, Melanoma B 16, cervical squamous carcinoma HeLa, Chinese hamster V79 and hepatoma SMMC-7721. Cell survival was determined by a colonogenic assay, and the sensitivity was described by D(50) (the dose of radiation necessary to reduce the survival to 50%). For all cell lines, D(50) ranged from 0.74 Gy to 3.85 Gy, among them B 16 was the most radiosensitive to (12)C ions, and V79 and HeLa cells had almost the same radio-sensitivity, SMMC-7721 was the last. The induction of deproteinized DNA double-strand breaks induced by (12)C ions were measured by pulsed-field gel electrophoresis (PFGE), and the initial yield of the deproteinized DNA dsbs per unit dose was expressed as the DNA double break level (L). A linear dose-response curve was seen for initial DNA dsbs for all cell lines (slopes range from 0.40-0.98 (DSBs/100Mbp/Gy)). V79 was the steepest, B 16 was the last, There was an inverse relationship between the initial DNA dsb and D(50) if the B 16 cell line was not considered, but there was no relativity even excludes the B16 cell line. The present results indicate that there is no relationship between cellular sensitivity and initial DNA dsb, even exclude the effects of chromatin structure. |
| WOS关键词 | DOUBLE-STRAND BREAKS ; CHEF ELECTROPHORESIS ; GEL-ELECTROPHORESIS ; V79 CELLS ; RADIOSENSITIVITY ; RADIATION ; LINES ; CHROMATIN ; REPAIR ; INDUCTION |
| 资助项目 | National Natural Science Foundation of China[10875153] |
| WOS研究方向 | Nuclear Science & Technology ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000274566600007 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://ir.impcas.ac.cn/handle/113462/18592]  |
| 专题 | 近代物理研究所_医学物理研究室 近代物理研究所_生物物理研究室 |
| 通讯作者 | Li Wenjian |
| 作者单位 | Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Qiang,Wang Jufang,Li Wenjian,et al. Relationship between cellular radio-sensitivity and naked DNA damage in mammalian cells exposed to heavy ions[J]. NUCLEAR SCIENCE AND TECHNIQUES,2009,20:349-353. |
| APA | Li Qiang,Wang Jufang,Li Wenjian,Zhou Guangming,Wang Zhuanzi,&Dang Bingrong.(2009).Relationship between cellular radio-sensitivity and naked DNA damage in mammalian cells exposed to heavy ions.NUCLEAR SCIENCE AND TECHNIQUES,20,349-353. |
| MLA | Li Qiang,et al."Relationship between cellular radio-sensitivity and naked DNA damage in mammalian cells exposed to heavy ions".NUCLEAR SCIENCE AND TECHNIQUES 20(2009):349-353. |
入库方式： OAI收割
来源：近代物理研究所
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