Heavy ion beams induce survivin expression in human hepatoma SMMC-7721 cells more effectively than x-rays
文献类型：期刊论文
| 作者 | Li, Qiang ; An, Lizhe; Gong, Li; Jin, Xiaodong; Liu, Jiangtao
|
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2007-08-01 |
| 卷号 | 39页码:575-582 |
| 关键词 | survivin human hepatoma SMMC-7721 cell high-LET carbon ion low-LET X-ray radiosensitivity |
| ISSN号 | 1672-9145 |
| DOI | 10.1111/j.1745-7270.2007.00314.x |
| 英文摘要 | High linear energy transfer (LET) heavy ion radiation is more effective in inducing biological damage than low-LET X-rays or gamma-rays. Heavy ion beam provides good dose localization (Bragg peak) in critical cancer tissue and gives higher relative biological effectiveness in cell killing across the dose peak, so high-LET heavy ion beam is superior to low-LET radiation in cancer treatment. Survivin, as a member of the inhibitor of apoptosis protein family, might help cancerous cells to overcome the G(2)/M apoptotic checkpoint and favor the aberrant progression of transformed cells through mitosis. Survivin expression in the human hepatoma SMMC-7721 cell line after exposure to low-LET X-ray and high-LET carbon ion irradiation was investigated in this study. Compared with X-ray irradiation, the carbon ion beam clearly caused G(2)/M arrest and promoted the expression of the survivin gene in a dose-dependent manner. Clonogenic survival assay showed that SMMC-7721 cells were more radiosensitive to the high-LET carbon ions than to the X-rays, and the radiosensitivity was promoted after treatment with specific survivin short interfering RNA. Differential survivin expression at both transcriptional and translational levels was found for SMMC-7721 cells following low- and high-LET irradiation. The overexpression of survivin in SMMC-7721 cells is probably an important reason why the cancerous cells have radioresistance to strong stimulus such as dense ionizing high-LET radiation. However, the direct killing effect on cancerous cells by high-LET radiation might be more significant than the apoptosis inhibition through the overexpression of survivin following heavy ion irradiation. |
| WOS关键词 | LUNG-CANCER ; RADIORESISTANCE FACTOR ; THERAPEUTIC TARGET ; PANCREATIC-CANCER ; INDUCED APOPTOSIS ; RADIOTHERAPY ; RADIOSENSITIVITIES ; CHEMOTHERAPY ; CARCINOMAS ; ADRIAMYCIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000249205600004 |
| 出版者 | BLACKWELL PUBLISHING |
| 源URL | [http://ir.impcas.ac.cn/handle/113462/18593]  |
| 专题 | 近代物理研究所_医学物理研究室 近代物理研究所_生物物理研究室 |
| 通讯作者 | An, Lizhe |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, Lanzhou 370000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 370000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Qiang,An, Lizhe,Gong, Li,et al. Heavy ion beams induce survivin expression in human hepatoma SMMC-7721 cells more effectively than x-rays[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2007,39:575-582. |
| APA | Li, Qiang,An, Lizhe,Gong, Li,Jin, Xiaodong,&Liu, Jiangtao.(2007).Heavy ion beams induce survivin expression in human hepatoma SMMC-7721 cells more effectively than x-rays.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,39,575-582. |
| MLA | Li, Qiang,et al."Heavy ion beams induce survivin expression in human hepatoma SMMC-7721 cells more effectively than x-rays".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 39(2007):575-582. |
入库方式： OAI收割
来源：近代物理研究所
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