Toxic effects and foundation of proton radiation on the early-life stage of zebrafish development
文献类型：期刊论文
| 作者 | Zhao, Baoquan2; Hu, Burong3,4 ; Jia, Rong1,3,4; Wang, Yongan2; Zha, Xiaodan2; Li, Xiaoman1,2,3,4
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| 刊名 | CHEMOSPHERE
 |
| 出版日期 | 2018-06-01 |
| 卷号 | 200页码:302-312 |
| 关键词 | Proton beam radiation Development of zebrafish Toxic effects Gene expression Mitochondrial dysfunction |
| ISSN号 | 0045-6535 |
| DOI | 10.1016/j.chemosphere.2018.02.141 |
| 英文摘要 | Proton is a major particle of space radiation environment and a prospective radiotherapy beam. However, its risk needs to be fully evaluated for the understanding and to establish the better protective strategy for astronaut and patient. Zebrafish is an ideal model for the toxicity studies on medicines and environmental genetic toxicants. In the current study, embryos of zebrafish at 24 h post-fertilization (hpf) were exposed to proton beam. Some toxic parameters of embryo-larval development were investigated. Microarray combining with qRT-PCR were used to detect the gene expression situation. Generally, fractions of a variety of abnormal phenotypes of embryos and larvae increased in a dose-dependent manner after irradiation. The copy number of mitochondria, the basal respiration rate and the maximum respiration rate of embryos significantly decreased after irradiation. Microarray data demonstrated that MAPK signaling pathway, cell communication, glycolysis and TGF-beta signaling pathway were significantly affected in the irradiated group. The expressions of matrix metallopeptidase 9 (mmp9) and TIMP metallopeptidase inhibitor 2b (timp2b) genes, and enzymatic activity of MMP9 were significantly upregulated in irradiated group. Overall, these results suggest that acute radiation of proton severely affects the development of organism and results in aberration occurrence in the early stage of zebrafish development, which may relates to mitochondrial and glycolytic dysfunction. (C) 2018 Elsevier Ltd. All rights reserved. |
| WOS关键词 | DANIO-RERIO ; OXIDATIVE STRESS ; GENE-EXPRESSION ; SPACE RADIATION ; EMBRYO DEVELOPMENT ; ION RADIATION ; BEAM THERAPY ; CELLS ; ACTIVATION ; MICE |
| 资助项目 | National Key Scientific Instrument and Equipment Development Project of China[2012YQ03014211] ; National Key Scientific Instrument and Equipment Development Project of China[2012YQ03014210] ; National Natural Science Foundation of China[81173572] ; National Natural Science Foundation of China[11635013] ; Foundation of General Logistics Department of PLA[DWS16J007] ; Military Medical Scientific and Technological Project for the |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:000429891300034 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 资助机构 | National Key Scientific Instrument and Equipment Development Project of China ; National Natural Science Foundation of China ; Foundation of General Logistics Department of PLA ; Military Medical Scientific and Technological Project for the |
| 源URL | [http://119.78.100.186/handle/113462/24432]  |
| 专题 | 近代物理研究所_空间辐射生物研究室 |
| 通讯作者 | Zhao, Baoquan; Hu, Burong |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Acad Mil Med Sci, Inst Pharmacol & Toxicol, State Key Lab Toxicol & Med Countermeasures, Beijing 100850, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, CAS Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Gansu, Peoples R China 4.Chinese Acad Sci, Inst Modern Phys, Key Lab Space Radiobiol Gansu Prov, Lanzhou 730000, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Baoquan,Hu, Burong,Jia, Rong,et al. Toxic effects and foundation of proton radiation on the early-life stage of zebrafish development[J]. CHEMOSPHERE,2018,200:302-312. |
| APA | Zhao, Baoquan,Hu, Burong,Jia, Rong,Wang, Yongan,Zha, Xiaodan,&Li, Xiaoman.(2018).Toxic effects and foundation of proton radiation on the early-life stage of zebrafish development.CHEMOSPHERE,200,302-312. |
| MLA | Zhao, Baoquan,et al."Toxic effects and foundation of proton radiation on the early-life stage of zebrafish development".CHEMOSPHERE 200(2018):302-312. |
入库方式： OAI收割
来源：近代物理研究所
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