Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells
文献类型：期刊论文
| 作者 | Chen, Yaxiong4,5 ; Du, Yarong4,5; Ren, Zhenxin4,5; Li, Xiaoman1,4,5; Hu, Burong2,3,4,5; Pan, Dong1,4,5
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| 刊名 | ONCOTARGET
 |
| 出版日期 | 2017 |
| 卷号 | 8页码:4422-4435 |
| ISSN号 | 1949-2553 |
| 关键词 | three dimensional cultured cells radioresistance methylation promoter RBL1 |
| DOI | 10.18632/oncotarget.12647 |
| 英文摘要 | Three dimensional (3D) culture in vitro is a new cell culture model that more closely mimics the physiology features of the in vivo environment and is being used widely in the field of medical and biological research. It has been demonstrated that cancer cells cultured in 3D matrices are more radioresistant compared with cells in monolayer (2D). However, the mechanisms causing this difference remain largely unclear. Here we found that the cell cycle distribution and expression of cell cycle regulation genes in 3D A549 cells are different from the 2D. The higher levels of the promotor methylation of cell cycle regulation genes such as RBL1 were observed in 3D A549 cells compared with cells in 2D. The treatments of irradiation or 5-Aza-CdR activated the demethylation of RBL1 promotor and resulted in the increased expression of RBL1 only in 3D A549 cells. Inhibition of RBL1 enhanced the radioresistance and decreased the G2/M phase arrest induced by irradiation in 2D A549 and MCF7 cells. Overexpression of RBL1 sensitized 3D cultured A549 and MCF7 cells to irradiation. Taken together, to our knowledge, it is the first time to revealthat the low expression of RBL1 due to itself promotor methylation in 3D cells enhances the radioresistance. Our finding sheds a new light on understanding the features of the 3D cultured cell model and its application in basic research into cancer radiotherapy and medcine development. |
| WOS关键词 | MAMMARY EPITHELIAL-CELLS ; DNA METHYLATION ; GENE-EXPRESSION ; EXTRACELLULAR-MATRIX ; RADIATION SENSITIVITY ; TISSUE ARCHITECTURE ; CANCER STATISTICS ; BREAST-CANCER ; P107 PROTEIN ; HYPERMETHYLATION |
| 资助项目 | National Natural Science Foundation of China[U1432121] ; National Natural Science Foundation of China[11635013] ; National Natural Science Foundation of China[31170803] ; Major Project Specialized for Infectious Diseases of the Chinese Health and Family Planning Commission[2014ZX10004002] ; National Key Scientific Instrument and Equipment Development Project of China[2012YQ03014210] |
| WOS研究方向 | Oncology ; Cell Biology |
| 语种 | 英语 |
| 出版者 | IMPACT JOURNALS LLC |
| WOS记录号 | WOS:000393228400056 |
| 资助机构 | National Natural Science Foundation of China ; Major Project Specialized for Infectious Diseases of the Chinese Health and Family Planning Commission ; National Key Scientific Instrument and Equipment Development Project of China |
| 源URL | [http://119.78.100.186/handle/113462/44286]  |
| 专题 | 近代物理研究所_空间辐射生物研究室 |
| 通讯作者 | Hu, Burong |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Soochow Univ, Collaborat Innovat Ctr Radiat Med Jiangsu Higher, Suzhou, Jiangsu, Peoples R China 3.Soochow Univ, Sch Radiol & Interdisciplinary Sci RAD X, Suzhou, Jiangsu, Peoples R China 4.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China 5.Chinese Acad Sci, Inst Modern Phys, Key Lab Space Radiobiol Gansu Prov, Lanzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Yaxiong,Du, Yarong,Ren, Zhenxin,et al. Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells[J]. ONCOTARGET,2017,8:4422-4435. |
| APA | Chen, Yaxiong,Du, Yarong,Ren, Zhenxin,Li, Xiaoman,Hu, Burong,&Pan, Dong.(2017).Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells.ONCOTARGET,8,4422-4435. |
| MLA | Chen, Yaxiong,et al."Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells".ONCOTARGET 8(2017):4422-4435. |
入库方式： OAI收割
来源：近代物理研究所
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