Quantitative proteomic analysis for radiation-induced cell cycle suspension in 92-1 melanoma cell line
文献类型：期刊论文
| 作者 | Zhang, Yanan1 ; Wang, Fengling1; Bing, Zhitong1; Ao, Bin1; Zhang, Sheng1,2; Ye, Caiyong1,2; He, Jinpeng1,2; Ding, Nan1,2; Ye, Wenling1; Xiong, Jie1
|
| 刊名 | JOURNAL OF RADIATION RESEARCH
 |
| 出版日期 | 2013-07-01 |
| 卷号 | 54页码:649-662 |
| 关键词 | Radiation Uveal Melanoma 2d-lc-ms/ms Go Biological Pathway |
| ISSN号 | 0449-3060 |
| DOI | 10.1093/jrr/rrt010 |
| 文献子类 | Article |
| 英文摘要 | Melanoma is a malignant tumor with high invasive and metastatic properties. Though radiation is the major therapy for melanoma, its radio-resistance has been shown to severely influence the clinical outcome. So it is imperative to enhance the sensitivity of uveal melanoma cells to radiotherapy. Previously, we found that the cell cycle of 92-1 uveal melanoma cells was suspended and remained unchanged for up to 5 days after exposure to 10 Gy of X-rays, which might be relevant to the high radio-sensitivity of 92-1 cells. To further investigate the cell cycle suspension-associated proteins, we employed two analyses with stable isotope labeling with amino acids in cell culture technology and two-dimensional liquid chromatography tandem mass spectrometry. Cells were incubated for 15 h or 48 h after irradiation with 10 Gy of X-rays. We identified a total of 737 proteins at 15 h (Group A) and 530 proteins at 48 h post-irradiation (Group B). The gene ontology biological pathway was used to obtain a systems level view of proteome changes in 92-1cells under cell cycle suspension. We further selected the significantly changed proteins for investigation of their potential contribution to cell cycle suspension, growth arrest and cell senescence. These proteins are involved in the cell cycle, stress response, glycolysis and the tricarboxylic acid cycle, etc. Our study expected to reveal potential marker proteins associated with cell suspension induced by irradiation, which might contribute to understanding the mechanism beyond the cell cycle suspension. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; UVEAL MELANOMA ; FUNCTIONAL-ANALYSIS ; MALIGNANT-MELANOMA ; PROTEIN ; GENE ; APOPTOSIS ; SENESCENCE ; EXPRESSION ; THERAPY |
| 资助项目 | Foundation for Young Talents of Gansu[1208RJYA013] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000321769500007 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://119.78.100.186/handle/113462/48391]  |
| 专题 | 近代物理研究所_计算物理研究室 近代物理研究所_空间辐射生物研究室 |
| 通讯作者 | Zhou, Guangming |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Natl Inst Radiol Sci, Res Ctr Charged Particle Therapy, Chiba 263555, Japan |
| 推荐引用方式 GB/T 7714 | Zhang, Yanan,Wang, Fengling,Bing, Zhitong,et al. Quantitative proteomic analysis for radiation-induced cell cycle suspension in 92-1 melanoma cell line[J]. JOURNAL OF RADIATION RESEARCH,2013,54:649-662. |
| APA | Zhang, Yanan.,Wang, Fengling.,Bing, Zhitong.,Ao, Bin.,Zhang, Sheng.,...&Yang, Lei.(2013).Quantitative proteomic analysis for radiation-induced cell cycle suspension in 92-1 melanoma cell line.JOURNAL OF RADIATION RESEARCH,54,649-662. |
| MLA | Zhang, Yanan,et al."Quantitative proteomic analysis for radiation-induced cell cycle suspension in 92-1 melanoma cell line".JOURNAL OF RADIATION RESEARCH 54(2013):649-662. |
入库方式： OAI收割
来源：近代物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。