Study of a High-Yield Cellulase System Created by Heavy-Ion Irradiation-Induced Mutagenesis of Aspergillus niger and Mixed Fermentation with Trichoderma reesei
文献类型：期刊论文
| 作者 | Jiang, Bo-Ling1,2; Wang, Shu-Yang2,3 ; Zhou, Xiang2; Chen, Ji-Hong2; Li, Wen-Jian2; Liu, Jing2; Hu, Wei2; Xiao, Guo-Qing2; Dong, Miao-Yin1,2; Wang, Yu-Chen2
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| 刊名 | PLOS ONE
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| 出版日期 | 2015-12-11 |
| 卷号 | 10页码:15 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0144233 |
| 英文摘要 | The aim of this study was to evaluate and validate the efficiency of C-12(6+) irradiation of Aspergillus niger (A. niger) or mutagenesis via mixed Trichoderma viride (T. viride) culturing as well as a liquid cultivation method for cellulase production via mixed Trichoderma reesei (T. reesei) and A. niger culture fermentation. The first mutagenesis approach was employed to optimize yield from a cellulase-producing strain via heavy-ion mutagenesis and high-throughput screening, and the second was to effectively achieve enzymatic hydrolysis of cellulase from a mixed culture of mutant T. viride and A. niger. We found that C-12(6+)-ion irradiation induced changes in cellulase biosynthesis in A. niger but had no effect on the time course of the synthesis. It is notable that the exoglucanases (CBH) activities of A. niger strains H11-1 and H differed (6.71 U/mL vs. 6.01 U/mL) and were significantly higher than that of A. niger mutant H3-1. Compared with strain H, the filter paper assay (FPA), endoglucanase (EG) and beta-glucosidase (BGL) activities of mutant strain H11-1 were increased by 250.26%, 30.26% and 34.91%, respectively. A mixed culture system was successfully optimized, and the best ratio of T. reesei to A. niger was 5: 1 for 96 h with simultaneous inoculation. The BGL activity of the mixed culture increased after 72 h. At 96 h, the FPA and BGL activities of the mixed culture were 689.00 and 797.15 U/mL, respectively, significantly higher than those of monocultures, which were 408.70 and 646.98 U/mL for T. reesei and 447.29 and 658.89 U/mL for A. niger, respectively. The EG activity of the mixed culture was 2342.81 U/mL, a value that was significantly higher than that of monocultures at 2206.57 U/mL for T. reesei and 1727.62 U/mL for A. niger. In summary, cellulose production and hydrolysis yields were significantly enhanced by the proposed combination scheme. |
| WOS关键词 | EXPLODED CORN STOVER ; ENZYMATIC-HYDROLYSIS ; ETHANOL-PRODUCTION ; NEUROSPORA-CRASSA ; CELLULOSIC ETHANOL ; BETA-GLUCOSIDASE ; WHEAT-STRAW ; BIO-ETHANOL ; RUT-C30 ; OPTIMIZATION |
| 资助项目 | National Natural Science Foundation of China[11305225] ; National Natural Science Foundation of China[11105193] ; Science and Technology Service Network Initiative[KFJ-EW-STS-086] ; Western Action Plan of CCAS[kzcxz-xb3-05] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| WOS记录号 | WOS:000366903600031 |
| 资助机构 | National Natural Science Foundation of China ; Science and Technology Service Network Initiative ; Western Action Plan of CCAS |
| 源URL | [http://ir.impcas.ac.cn/handle/113462/23331]  |
| 专题 | 近代物理研究所_生物物理研究室 近代物理研究所_兰州重离子研究装置 |
| 通讯作者 | Wang, Shu-Yang |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 3.Lanzhou Univ, Lanzhou 730000, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Bo-Ling,Wang, Shu-Yang,Zhou, Xiang,et al. Study of a High-Yield Cellulase System Created by Heavy-Ion Irradiation-Induced Mutagenesis of Aspergillus niger and Mixed Fermentation with Trichoderma reesei[J]. PLOS ONE,2015,10:15. |
| APA | Jiang, Bo-Ling.,Wang, Shu-Yang.,Zhou, Xiang.,Chen, Ji-Hong.,Li, Wen-Jian.,...&Wang, Yu-Chen.(2015).Study of a High-Yield Cellulase System Created by Heavy-Ion Irradiation-Induced Mutagenesis of Aspergillus niger and Mixed Fermentation with Trichoderma reesei.PLOS ONE,10,15. |
| MLA | Jiang, Bo-Ling,et al."Study of a High-Yield Cellulase System Created by Heavy-Ion Irradiation-Induced Mutagenesis of Aspergillus niger and Mixed Fermentation with Trichoderma reesei".PLOS ONE 10(2015):15. |
入库方式： OAI收割
来源：近代物理研究所
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