A high-throughput screening method for breeding Aspergillus niger with C-12(6+) ion beam-improved cellulase
文献类型：期刊论文
| 作者 | Liu, Jing1 ; Li, Wen-Jian1; Chen, Ji-Hong1; Xiao, Guo-Qing1; Wang, Shu-Yang1,3; Jiang, Bo-Ling1,4; Wang, Yu-Chen1,2; Dong, Miao-Ying1; Xu, Fu-Qiang1; Hu, Wei1
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| 刊名 | NUCLEAR SCIENCE AND TECHNIQUES
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| 出版日期 | 2017 |
| 卷号 | 28页码:7 |
| 关键词 | High-throughput screening method Aspergillus niger H11 Cellulase C-12(6+) ion beam |
| ISSN号 | 1001-8042 |
| DOI | 10.1007/s41365-016-0157-8 |
| 英文摘要 | In this study, a high-throughput screening method was established through the 24-square deep-well microliter plate (MTP) fermentation and micro-plate detection for large-scale screening of the mutants. It was suitable for screening a large number of mutants and improving the breeding efficiency after heavy-ion beam irradiation. Seventeen strains showed higher cellulase activity compared with the initial strain after the screening of plate and MTP fermentation. The filter paper activity and beta-glucosidase activity of Aspergillus niger H11201 had increased 38.74 and 63.23 % separately compared with A. niger H11 by shaking flask fermentation, and it was genetically stable after being passaged to nine generations. The results indicate that the high-throughput screening method can be used for the quick breeding of A. niger with high cellulase activity. |
| WOS关键词 | IRRADIATION ; REPRESSION ; INDUCTION ; ASSAY |
| 资助项目 | National Natural Science Foundation of China[11305225] ; Science and Technology Service Network Initiative[KFJ-EW-STS-086] |
| WOS研究方向 | Nuclear Science & Technology ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000393150100004 |
| 出版者 | SPRINGER SINGAPORE PTE LTD |
| 资助机构 | National Natural Science Foundation of China ; Science and Technology Service Network Initiative |
| 源URL | [http://ir.impcas.ac.cn/handle/113462/23528]  |
| 专题 | 近代物理研究所_生物物理研究室 近代物理研究所_兰州重离子研究装置 |
| 通讯作者 | Wang, Shu-Yang |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Peoples R China 2.Gansu Agr Univ, Lanzhou 730000, Gansu, Peoples R China 3.Lanzhou Univ, 222 South Tianshui Rd, Lanzhou 730000, Gansu, Peoples R China 4.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jing,Li, Wen-Jian,Chen, Ji-Hong,et al. A high-throughput screening method for breeding Aspergillus niger with C-12(6+) ion beam-improved cellulase[J]. NUCLEAR SCIENCE AND TECHNIQUES,2017,28:7. |
| APA | Liu, Jing.,Li, Wen-Jian.,Chen, Ji-Hong.,Xiao, Guo-Qing.,Wang, Shu-Yang.,...&Hu, Wei.(2017).A high-throughput screening method for breeding Aspergillus niger with C-12(6+) ion beam-improved cellulase.NUCLEAR SCIENCE AND TECHNIQUES,28,7. |
| MLA | Liu, Jing,et al."A high-throughput screening method for breeding Aspergillus niger with C-12(6+) ion beam-improved cellulase".NUCLEAR SCIENCE AND TECHNIQUES 28(2017):7. |
入库方式： OAI收割
来源：近代物理研究所
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