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Heavy ion mutagenesis combined with triclosan screening provides a new strategy for improving the arachidonic acid yield in Mortierella alpina

文献类型：期刊论文

作者Zhang, Huidan2,4,5; Lu, Dong3; Li, Xin3; Feng, Yingang2,5; Cui, Qiu1,2,5; Song, Xiaojin2,5
刊名BMC BIOTECHNOLOGY
出版日期2018-05-02
卷号18期号:1页码:23
ISSN号1472-6750
关键词Arachidonic acid Desaturase Heavy ion mutagenesis Mortierella alpina Octyl gallate Triclosan
DOI10.1186/s12896-018-0437-y
英文摘要Background: Arachidonic acid (ARA), which is omega-6 polyunsaturated fatty acid, has a wide range of biological activities and is an essential component of cellular membranes in some human tissues. Mortierella alpina is the best strain for industrial production of ARA. To increase its yield of arachidonic acid, heavy ion beam irradiation mutagenesis of Mortierella alpina was carried out in combination with triclosan and octyl gallate treatment. Results: The obtained mutant strain F-23 ultimately achieved an ARA yield of 5.26 g L-1, which is 3.24 times higher than that of the wild-type strain. In addition, quantitative real-time PCR confirmed that the expression levels of fatty acid synthase (FAS), Delta 5-desaturase, Delta 6-desaturase, and Delta 9-desaturase were all significantly up-regulated in the mutant F-23 strain, especially Delta 6- and Delta 9-desaturase, which were up-regulated 3- and 2-fold, respectively. Conclusions: This study confirmed a feasible mutagenesis breeding strategy for improving ARA production and provided a mutant of Mortierella alpina with high ARA yield.
WOS关键词RICH OIL PRODUCTION ; OCTYL-GALLATE ; FERMENTATION STRATEGY ; STRAIN IMPROVEMENT ; ALKYL-GALLATE ; FUNGUS ; 1S-4 ; 5-FLUOROURACIL ; TRANSFORMATION ; IRRADIATION
资助项目National Key Research and Development Program[2016YFA0601400] ; Key research and development program of Shandong Province[2017YYSP026] ; Qingdao independent innovation major project[16-7-1-11-zdzx-nsh] ; Shandong Provincial Key Laboratory of Energy Genetics
WOS研究方向Biotechnology & Applied Microbiology
语种英语
出版者BIOMED CENTRAL LTD
WOS记录号WOS:000431276200001
资助机构National Key Research and Development Program ; Key research and development program of Shandong Province ; Qingdao independent innovation major project ; Shandong Provincial Key Laboratory of Energy Genetics
源URL[http://119.78.100.186/handle/113462/24469]  
专题近代物理研究所_生物物理研究室
通讯作者Song, Xiaojin
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China
3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Qingdao Engn Lab Single Cell Oil, Qingdao 266101, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Huidan,Lu, Dong,Li, Xin,et al. Heavy ion mutagenesis combined with triclosan screening provides a new strategy for improving the arachidonic acid yield in Mortierella alpina[J]. BMC BIOTECHNOLOGY,2018,18(1):23.
APA Zhang, Huidan,Lu, Dong,Li, Xin,Feng, Yingang,Cui, Qiu,&Song, Xiaojin.(2018).Heavy ion mutagenesis combined with triclosan screening provides a new strategy for improving the arachidonic acid yield in Mortierella alpina.BMC BIOTECHNOLOGY,18(1),23.
MLA Zhang, Huidan,et al."Heavy ion mutagenesis combined with triclosan screening provides a new strategy for improving the arachidonic acid yield in Mortierella alpina".BMC BIOTECHNOLOGY 18.1(2018):23.

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来源：近代物理研究所

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