Identification of heavy-ion radiation-induced microRNAs in rice
文献类型：期刊论文
| 作者 | Shi, Jinming2; Huang, Lei3; Sun, Yeqing2,3; Cheng, Zhenlong3; Xiang, Yingxia3; Li, Wenjian1 ; Zhang, Meng2; Liang, Shujian2; Hang, Xiaoming3
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| 刊名 | ADVANCES IN SPACE RESEARCH
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| 出版日期 | 2011-03-15 |
| 卷号 | 47页码:1054-1061 |
| 关键词 | Rice Radiation MicroRNA miR156 miR164 |
| ISSN号 | 0273-1177 |
| DOI | 10.1016/j.asr.2010.10.024 |
| 英文摘要 | MicroRNAs (miRNAs) are a family of small non-coding RNAs, which play significant roles in regulating development and stress responses in plant. As an excellent model organism for studying the effects of environmental stress, rice has been used to assess the damage of the space radiation environment for decades. Heavy-ions radiation show higher relative biological effectiveness compared to other cosmic-rays radiation. To identify the specific miRNAs that underlie biological effects of heavy-ion radiation, the germinated seeds of rice were exposed to 1 Gy, 10 Gy and 20 Gy dose of C-12 heavy-ion radiation, respectively. Analysis of phenotype indicated that 20 Gy dose of heavy-ion radiation was the semi-lethal dose of rice seedling. The microarray of mu paraflo (TM) chip was employed to monitor the expression profiles of miRNAs in rice (Oryza saliva) under 20 Gy dose of radiation stress. miR164a, miR164c, miR164d and miR156a-j were identified as heavy-ion radiation-induced miRNAs. miR164 and miR156 family were increased in all three exposed samples by using quantitative real-time PCR (qRT-RCP). As targets of miR156 and miR164, SQUAMOSA PROMOTER BINDING-LIKE (SPL) transcription factors and NAM/ATAF/CUC (NAC) transcription factors expression were down-regulated correlating with an up-regulated level of the regulated miRNAs. Since SPL transcription factors and NAC transcription factors regulated growth and development of plant, we used 2-dimension electrophoresis (2-DE) gel to analyze changes of functional proteins in 20 Gy exposed samples. It was evident that both the height and survival rates of seedlings were markedly decreased. The abundance of some developmentally regulated proteins was also changed. To our knowledge, this study is the first to report heavy-ion radiation stress responsive miRNAs in plant. Moreover, our findings are important to understand the molecular mechanism of space biology. (C) 2010 COSPAR. Published by Elsevier Ltd. All rights reserved. |
| WOS关键词 | TRANSCRIPTION FACTOR FAMILY ; ARABIDOPSIS-THALIANA ; DIFFERENTIAL EXPRESSION ; GENOMIC INSTABILITY ; FUNCTIONAL-ANALYSIS ; IONIZING-RADIATION ; GENE-EXPRESSION ; AUXIN SIGNALS ; SMALL RNAS ; STRESS |
| 资助项目 | National High Technology Research and Development Program of China (863 Program)[2010AA7035020E] ; Demonstration Project of New Countryside Construction[2008E13SF185] |
| WOS研究方向 | Astronomy & Astrophysics ; Geology ; Meteorology & Atmospheric Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000288735800014 |
| 出版者 | ELSEVIER SCI LTD |
| 资助机构 | National High Technology Research and Development Program of China (863 Program) ; Demonstration Project of New Countryside Construction |
| 源URL | [http://119.78.100.186/handle/113462/33384]  |
| 专题 | 近代物理研究所_生物物理研究室 |
| 通讯作者 | Sun, Yeqing |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Harbin Inst Technol, Sch Astronaut, Harbin 150001, Peoples R China 3.Dalian Maritime Univ, Inst Environm Syst Biol, Dalian 116026, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Jinming,Huang, Lei,Sun, Yeqing,et al. Identification of heavy-ion radiation-induced microRNAs in rice[J]. ADVANCES IN SPACE RESEARCH,2011,47:1054-1061. |
| APA | Shi, Jinming.,Huang, Lei.,Sun, Yeqing.,Cheng, Zhenlong.,Xiang, Yingxia.,...&Hang, Xiaoming.(2011).Identification of heavy-ion radiation-induced microRNAs in rice.ADVANCES IN SPACE RESEARCH,47,1054-1061. |
| MLA | Shi, Jinming,et al."Identification of heavy-ion radiation-induced microRNAs in rice".ADVANCES IN SPACE RESEARCH 47(2011):1054-1061. |
入库方式： OAI收割
来源：近代物理研究所
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