Detection of Novel Human MiRNAs Responding to X-ray Irradiation
文献类型：期刊论文
| 作者 | Ding, Nan1,2,3 ; Wu, Xin1,3; He, Jinpeng3; Chang, Lei1,3; Hu, Wentao1,3; Li, Wenjian3; Wang, Jufang3; Wang, Tieshan2; Zhou, Guangming3
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| 刊名 | JOURNAL OF RADIATION RESEARCH
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| 出版日期 | 2011-07-01 |
| 卷号 | 52页码:425-432 |
| 关键词 | microRNA Ionizing radiation Solexa qRT-PCR |
| ISSN号 | 0449-3060 |
| DOI | 10.1269/jrr.10158 |
| 英文摘要 | Up to now, more than 1048 human miRNAs have been identified. However, the recognition of new human miRNAs is becoming more and more difficult. Based on the hypothesis that the expression of some miRNAs can be induced by ionizing radiation, total RNAs of HeLa cells were isolated 1 h after exposure to 2 Gy of X-rays, and total small RNAs were enriched and sequenced by PAGE and Solexa technology, respectively. As a result, 421 kinds of known miRNAs and 337 kinds of unknown sequences were identified, among which 10 novel miRNAs were characterized by bioinformatic approach and verified by qRT-PCR. Finally, putative targets of these miRNAs were predicted by TargetScan software and compared with known proteins down-regulated by radiation. it was confirmed that some of the targets of these novel miRNAs were radiation-related proteins. These results imply that these 10 novel miRNAs are radiation-related miRNAs. This study reveals a new way to find novel miRNAs. |
| WOS关键词 | EMBRYONIC STEM-CELLS ; MICRORNA EXPRESSION PATTERNS ; IONIZING-RADIATION ; SMALL RNAS ; CELLULAR STRESS ; ABIOTIC STRESS ; IDENTIFICATION ; CANCER ; BIOGENESIS ; THERAPY |
| 资助项目 | Chinese Academy of Sciences[O760140BRO] ; Chinese Academy of Sciences[KJCX2-YW-N34-2] ; Major State Basic Research Development Program of China (973 Program)[2010CB834201] ; National Natural Science Foundation of China[10979062] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000294885200004 |
| 出版者 | JAPAN RADIATION RESEARCH SOC |
| 资助机构 | Chinese Academy of Sciences ; Major State Basic Research Development Program of China (973 Program) ; National Natural Science Foundation of China |
| 源URL | [http://119.78.100.186/handle/113462/34665]  |
| 专题 | 近代物理研究所_生物物理研究室 |
| 通讯作者 | Zhou, Guangming |
| 作者单位 | 1.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 2.Lanzhou Univ, Sch Nucl Sci & Technol, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Nan,Wu, Xin,He, Jinpeng,et al. Detection of Novel Human MiRNAs Responding to X-ray Irradiation[J]. JOURNAL OF RADIATION RESEARCH,2011,52:425-432. |
| APA | Ding, Nan.,Wu, Xin.,He, Jinpeng.,Chang, Lei.,Hu, Wentao.,...&Zhou, Guangming.(2011).Detection of Novel Human MiRNAs Responding to X-ray Irradiation.JOURNAL OF RADIATION RESEARCH,52,425-432. |
| MLA | Ding, Nan,et al."Detection of Novel Human MiRNAs Responding to X-ray Irradiation".JOURNAL OF RADIATION RESEARCH 52(2011):425-432. |
入库方式： OAI收割
来源：近代物理研究所
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