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Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect

文献类型：期刊论文

作者Ding, Nan1; Zhu, Qiyun2; Wei, Wenjun1; Hua, Junrui1; Wang, Bing1; Zhang, Xurui1; Hu, Wentao1; Wang, Jufang1; Xu, Shuai1,2
刊名RNA BIOLOGY
出版日期2015-12-02
卷号12页码:1355-1363
关键词bystander effect exosome microRNA miR-21 radiation
ISSN号1547-6286
DOI10.1080/15476286.2015.1100795
英文摘要Bystander effects can be induced through cellular communication between irradiated cells and non-irradiated cells. The signals that mediate this cellular communication, such as cytokines, reactive oxygen species, nitric oxide and even microRNAs, can be transferred between cells via gap junctions or extracellular medium. We have previously reported that miR-21, a well described DDR (DNA damage response) microRNA, is involved in radiation-induced bystander effects through a medium-mediated way. However, the mechanisms of the microRNA transfer have not been elucidated in details. In the present study, it was found that exosomes isolated from irradiated conditioned medium could induce bystander effects. Furthermore, we demonstrated plenty of evidences that miR-21, which is up-regulated as a result of mimic transfection or irradiation, can be transferred from donor or irradiated cells into extracellular medium and subsequently get access to the recipient or bystander cells through exosomes to induce bystander effects. Inhibiting the miR-21 expression in advance can offset the bystander effects to some extent. From all of these results, it can be concluded that the exosome-mediated microRNA transfer plays an important role in the radiation-induced bystander effects. These findings provide new insights into the functions of microRNAs and the cellular communication between the directly irradiated cells and the non-irradiated cells.
WOS关键词IONIZING-RADIATION ; DIAGNOSTIC BIOMARKERS ; CELL COMMUNICATION ; CANCER ; RNA ; MICROVESICLES ; MECHANISM ; GROWTH ; SPLEEN
资助项目National Natural Science Foundation of China[31270895] ; National Natural Science Foundation of China[31400723] ; National Natural Science Foundation of China[11405235]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000368027500008
出版者TAYLOR & FRANCIS INC
资助机构National Natural Science Foundation of China
源URL[http://119.78.100.186/handle/113462/41407]  
专题近代物理研究所_生物物理研究室
通讯作者Wang, Jufang
作者单位1.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Gansu Key Lab Space Radiobiol, Lanzhou, Peoples R China
2.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou, Peoples R China
推荐引用方式
GB/T 7714		 Ding, Nan,Zhu, Qiyun,Wei, Wenjun,et al. Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect[J]. RNA BIOLOGY,2015,12:1355-1363.
APA Ding, Nan.,Zhu, Qiyun.,Wei, Wenjun.,Hua, Junrui.,Wang, Bing.,...&Xu, Shuai.(2015).Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect.RNA BIOLOGY,12,1355-1363.
MLA Ding, Nan,et al."Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect".RNA BIOLOGY 12(2015):1355-1363.

入库方式： OAI收割

来源：近代物理研究所

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