中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic

文献类型:期刊论文

作者Lu Dong1,3,4; Zhang Miao-Miao3,4; Zhang Wen-De2; Cao Guo-Zhen1,3,4; Miao Jian-Shun3,4; Li Wen-Jian1,3,4
刊名NUCLEAR SCIENCE AND TECHNIQUES
出版日期2015-12-01
卷号26页码:9
关键词X-rays Saccharomyces cerevisiae Growth curve Physiological-biochemical characteristic
ISSN号1001-8042
英文摘要In this paper, the growth curves of yeast cells exposed to X-rays were detected, and then fitted by Gompertz equation. The yeast cells treated with 50-125 Gy showed an increased exponential growth rate, and lower total biomass at plateau. At doses >= 150 Gy, cells showed a decreased exponential growth rate and higher total biomass at plateau. DNA lesions were detected by comet assay. Meanwhile, intracellular accumulation of reactive oxygen species (ROS), reduction of mitochondrial membrane potential (Delta Psi m) and cell membrane integrity were evaluated. We conclude that X-ray irradiation results in DNA lesions, ROS accumulation and Delta Psi m decline in a dose-dependent manner, and that these changes may be one of causes of X-rays-induced apoptosis in yeast. Furthermore, yeast cell membrane integrity appeared compromised following irradiation, suggesting that membrane damage may also have a role in the biological effects of radiation.
WOS关键词IONIZING-RADIATION ; DEPENDENT APOPTOSIS ; TRACK STRUCTURE ; YEAST ; DNA ; MEMBRANE ; STRESS ; RESPONSES ; REPAIR ; DAMAGE
资助项目project of western talent training program of Chinese Academy of Sciences[Y306010XB0]
WOS研究方向Nuclear Science & Technology ; Physics
语种英语
WOS记录号WOS:000368964600008
出版者ELSEVIER SCIENCE BV
资助机构project of western talent training program of Chinese Academy of Sciences
源URL[http://119.78.100.186/handle/113462/41538]  
专题近代物理研究所_生物物理研究室
通讯作者Lu Dong
作者单位1.Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China
2.Gansu Ronghua Ind Grp Co Ltd, Wuwei 733000, Peoples R China
3.Key Lab Microbial Resources Exploitat & Applicat, Lanzhou 730000, Peoples R China
4.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714
Lu Dong,Zhang Miao-Miao,Zhang Wen-De,et al. Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic[J]. NUCLEAR SCIENCE AND TECHNIQUES,2015,26:9.
APA Lu Dong,Zhang Miao-Miao,Zhang Wen-De,Cao Guo-Zhen,Miao Jian-Shun,&Li Wen-Jian.(2015).Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic.NUCLEAR SCIENCE AND TECHNIQUES,26,9.
MLA Lu Dong,et al."Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic".NUCLEAR SCIENCE AND TECHNIQUES 26(2015):9.

入库方式: OAI收割

来源:近代物理研究所

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