Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic
文献类型：期刊论文
| 作者 | Lu Dong1,3,4; Zhang Miao-Miao3,4 ; Zhang Wen-De2; Cao Guo-Zhen1,3,4; Miao Jian-Shun3,4; Li Wen-Jian1,3,4
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| 刊名 | NUCLEAR SCIENCE AND TECHNIQUES
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 26页码:9 |
| 关键词 | X-rays Saccharomyces cerevisiae Growth curve Physiological-biochemical characteristic |
| ISSN号 | 1001-8042 |
| 英文摘要 | In this paper, the growth curves of yeast cells exposed to X-rays were detected, and then fitted by Gompertz equation. The yeast cells treated with 50-125 Gy showed an increased exponential growth rate, and lower total biomass at plateau. At doses >= 150 Gy, cells showed a decreased exponential growth rate and higher total biomass at plateau. DNA lesions were detected by comet assay. Meanwhile, intracellular accumulation of reactive oxygen species (ROS), reduction of mitochondrial membrane potential (Delta Psi m) and cell membrane integrity were evaluated. We conclude that X-ray irradiation results in DNA lesions, ROS accumulation and Delta Psi m decline in a dose-dependent manner, and that these changes may be one of causes of X-rays-induced apoptosis in yeast. Furthermore, yeast cell membrane integrity appeared compromised following irradiation, suggesting that membrane damage may also have a role in the biological effects of radiation. |
| WOS关键词 | IONIZING-RADIATION ; DEPENDENT APOPTOSIS ; TRACK STRUCTURE ; YEAST ; DNA ; MEMBRANE ; STRESS ; RESPONSES ; REPAIR ; DAMAGE |
| 资助项目 | project of western talent training program of Chinese Academy of Sciences[Y306010XB0] |
| WOS研究方向 | Nuclear Science & Technology ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000368964600008 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | project of western talent training program of Chinese Academy of Sciences |
| 源URL | [http://119.78.100.186/handle/113462/41538]  |
| 专题 | 近代物理研究所_生物物理研究室 |
| 通讯作者 | Lu Dong |
| 作者单位 | 1.Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China 2.Gansu Ronghua Ind Grp Co Ltd, Wuwei 733000, Peoples R China 3.Key Lab Microbial Resources Exploitat & Applicat, Lanzhou 730000, Peoples R China 4.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu Dong,Zhang Miao-Miao,Zhang Wen-De,et al. Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic[J]. NUCLEAR SCIENCE AND TECHNIQUES,2015,26:9. |
| APA | Lu Dong,Zhang Miao-Miao,Zhang Wen-De,Cao Guo-Zhen,Miao Jian-Shun,&Li Wen-Jian.(2015).Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic.NUCLEAR SCIENCE AND TECHNIQUES,26,9. |
| MLA | Lu Dong,et al."Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic".NUCLEAR SCIENCE AND TECHNIQUES 26(2015):9. |
入库方式： OAI收割
来源：近代物理研究所
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