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Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger

文献类型：期刊论文

作者Li, Wen-jian1,2; Wu, Qing-feng2; Liu, Jing1,2; Hu, Wei1,2; Song, Yuan1,2; Chen, Ji-hong1,2; Wang, Shu-yang1,2
刊名JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
出版日期2016-04-01
卷号17页码:262-270
关键词Carbon ion irradiation Physiological properties Mutation Citric acid accumulation
ISSN号1673-1581
DOI10.1631/jzus.B1500120
英文摘要The objective of this work was to produce citric acid from corn starch using a newly isolated mutant of Aspergillus niger, and to analyze the relationship between changes in the physiological properties of A. niger induced by carbon ion irradiation and citric acid accumulation. Our results showed that the physiological characteristics of conidia in A. niger were closely related to citric acid accumulation and that lower growth rate and viability of conidia may be beneficial to citric acid accumulation. Using corn starch as a raw material, a high-yielding citric acid mutant, named HW2, was obtained. In a 10-L bioreactor, HW2 can accumulate 118.9 g/L citric acid with a residual total sugar concentration of only 14.4 g/L. This represented an 18% increase in citric acid accumulation and a 12.5% decrease in sugar utilization compared with the original strain.
WOS关键词TEMPERATURE PLASMA ARTP ; SOLID-STATE FERMENTATION ; STRAIN IMPROVEMENT ; ARABIDOPSIS-THALIANA ; MUTANT STRAIN ; MUTATION ; MORPHOLOGY ; STARCH ; GROWTH ; PRODUCTIVITY
资助项目Agriculture Science Technology Achievement Transformation Fund, China[2013GB24910680]
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Research & Experimental Medicine
语种英语
WOS记录号WOS:000374298000002
出版者ZHEJIANG UNIV
资助机构Agriculture Science Technology Achievement Transformation Fund, China
源URL[http://119.78.100.186/handle/113462/42389]  
专题近代物理研究所_生物物理研究室
通讯作者Li, Wen-jian; Chen, Ji-hong
作者单位1.Key Lab Microbial Resources Exploit & Applicat, Lanzhou 730000, Peoples R China
2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714		 Li, Wen-jian,Wu, Qing-feng,Liu, Jing,et al. Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2016,17:262-270.
APA Li, Wen-jian.,Wu, Qing-feng.,Liu, Jing.,Hu, Wei.,Song, Yuan.,...&Wang, Shu-yang.(2016).Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,17,262-270.
MLA Li, Wen-jian,et al."Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 17(2016):262-270.

入库方式： OAI收割

来源：近代物理研究所

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