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Pulsed electromagnetic fields stimulate osteogenic differentiation and maturation of osteoblasts by upregulating the expression of BMPRII localized at the base of primary cilium

文献类型：期刊论文

作者Zhou, Jian4; Shi, Wen-Gui5; Xie, Yan-Fang4; Chen, Ke-Ming4; Xian, Cory J.1; Wang, Ju-Fang5; Ma, Hui-Ping3; Wang, Ming-Gang2; Fang, Qing-Qing4; Li, Shao-Feng4
刊名BONE
出版日期2016-12-01
卷号93页码:22-32
关键词Pulsed electromagnetic fields Osteoblasts Primary cilia BMP-Smad1/5/8 signaling BMPRII
ISSN号8756-3282
DOI10.1016/j.bone.2016.09.008
英文摘要Pulsed electromagnetic fields (PEMFs) have been considered as a potential candidate for the prevention and treatment of osteoporosis, however, the mechanism of its action is still elusive. We have previously reported that 50 Hz 0.6 mT PEMF5 stimulate osteoblastic differentiation and mineralization in a primary cilium-dependent manner, but did not know the reason. In the current study, we found that the PEMFs promoted osteogenic differentiation and maturation of rat calvarial osteoblasts (ROBs) by activating bone morphogenetic protein BMPSmad1/5/8 signaling on the condition that primary cilia were normal. Further studies revealed that BMPRII, the primary binding receptor of BMP ligand, was readily and strongly upregulated by PEMF treatment and localized at the bases of primary cilia. Abrogation of primary cilia with small interfering RNA sequence targeting IFT88 abolished the PEMF-induced upregulation of BMPRII and its ciliary localization. Knockdown of BMPRII expression level with RNA interference had no effects on primary cilia but significantly decreased the promoting effect of PEMF5 on osteoblastic differentiation and maturation. These results indicated that PEMFs stimulate osteogenic differentiation and maturation of osteoblast by primary cilium-mediated upregulation of BMPRII expression and subsequently activation of BMP-Smad1/5/8 signaling, and that BMPRII is the key component linking primary cilium and BMP-Smad1/5/8 pathway. This study has thus revealed the molecular mechanism for the osteogenic effect of PEMFs. (C) 2016 Elsevier Inc. All rights reserved.
WOS关键词MORPHOGENETIC PROTEIN-RECEPTOR ; SIGNALING PATHWAYS ; BONE-CELLS ; OSTEOPOROSIS ; ENDOCYTOSIS ; ACTIVATION ; MECHANISMS ; OSTEOCYTES ; APOPTOSIS ; DISEASE
资助项目National Natural Sciences Foundation of China[81270963] ; National Natural Sciences Foundation of China[81471090] ; National Natural Sciences Foundation of China[81571847] ; International Science & Technology Cooperation Program of China[2015DFR30940] ; NHMRC Australia Senior Research Fellowship[1042105]
WOS研究方向Endocrinology & Metabolism
语种英语
WOS记录号WOS:000386987500003
出版者ELSEVIER SCIENCE INC
资助机构National Natural Sciences Foundation of China ; International Science & Technology Cooperation Program of China ; NHMRC Australia Senior Research Fellowship
源URL[http://119.78.100.186/handle/113462/43593]  
专题近代物理研究所_生物物理研究室
通讯作者Chen, Ke-Ming
作者单位1.Univ South Australia, Sansom Inst Hlth Res, Sch Pharm & Med Sci, Adelaide, SA 5001, Australia
2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China
3.CPLA, Lanzhou Command, Lanzhou Gen Hosp, Dept Pharm, Lanzhou 730050, Peoples R China
4.CPLA, Lanzhou Command, Lanzhou Gen Hosp, Inst Orthopaed, Lanzhou 730050, Peoples R China
5.Chinese Acad Sci, Inst Modern Phys, Gansu Key Lab Space Radiobiol, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Jian,Shi, Wen-Gui,Xie, Yan-Fang,et al. Pulsed electromagnetic fields stimulate osteogenic differentiation and maturation of osteoblasts by upregulating the expression of BMPRII localized at the base of primary cilium[J]. BONE,2016,93:22-32.
APA Zhou, Jian.,Shi, Wen-Gui.,Xie, Yan-Fang.,Chen, Ke-Ming.,Xian, Cory J..,...&Gao, Yu-Hai.(2016).Pulsed electromagnetic fields stimulate osteogenic differentiation and maturation of osteoblasts by upregulating the expression of BMPRII localized at the base of primary cilium.BONE,93,22-32.
MLA Zhou, Jian,et al."Pulsed electromagnetic fields stimulate osteogenic differentiation and maturation of osteoblasts by upregulating the expression of BMPRII localized at the base of primary cilium".BONE 93(2016):22-32.

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来源：近代物理研究所

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