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miR-185 enhances the inhibition of proliferation and migration induced by ionizing radiation in melanoma

文献类型：期刊论文

作者Tian, Ning1,4; He, Jinpeng1,5; Wang, Bing1,2; Li, He1,2; Lin, Sulan1,2; Hua, Junrui1,5; Wang, Jufang1,5; Feng, Xiu1,4; Jin, Liangliang3; Yang, Yanli3
刊名ONCOLOGY LETTERS
出版日期2017-04-01
卷号13页码:2442-2448
关键词melanoma radiosensitivity microRNA miR-185
ISSN号1792-1074
DOI10.3892/ol.2017.5699
英文摘要Melanoma is an aggressive malignancy that is increasingly common and exhibits a poor patient survival rate. Radiotherapy is the primary option for patients with melanoma, particularly those who are not candidates for surgery; however, the therapeutic effect is limited due to the relative radioresistance of melanoma to ionizing radiation (IR). It has been reported that microRNAs (miRNAs) serve a vital role in determining the radiosensitivity of tumors; however, little is known concerning the radiosensitization of melanoma using miRNA. In the present study, the radiosensitization effect of miRNA 185 (miR-185), which has been demonstrated to reduce renal cancer radioresistance, was investigated in B16 cells, a skin melanoma cell line derived from C57/BL mice, was investigated. Cell proliferation and scratch wound healing assays were used to determine the proliferative and migratory abilities of B16 cells. Annexin V/propidium iodide double staining was used to determine the apoptosis induced by IR. A tumor formation assay was performed to determine the radiosensitization effect of miR-185 on melanoma cells in vivo. Proliferation marker protein Ki-67, and hematoxylin and eosin staining were used to assess the proliferative activity and histological changes, respectively. The results of the present study demonstrated that miR-185 suppresses cellular proliferation and migration, and enhances IR-induced apoptosis, and the inhibition of proliferation and migration, in vitro and in vivo, which provides an insight into understanding the radiosensitization of melanoma using miRNA.
WOS关键词CELL-CYCLE ; HEPATOCELLULAR-CARCINOMA ; CANCER CELLS ; MALIGNANT-MELANOMA ; COLORECTAL-CANCER ; GASTRIC-CANCER ; IN-VITRO ; EXPRESSION ; INVASION ; PATHWAY
资助项目National Nature Science Foundation of China[31270895] ; National Nature Science Foundation of China[11405232] ; National Nature Science Foundation of China[31400723]
WOS研究方向Oncology
语种英语
WOS记录号WOS:000398514200063
出版者SPANDIDOS PUBL LTD
资助机构National Nature Science Foundation of China
源URL[http://119.78.100.186/handle/113462/44635]  
专题近代物理研究所_生物物理研究室
通讯作者Wang, Jufang
作者单位1.Chinese Acad Sci, Gansu Key Lab Space Radiobiol, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Lanzhou Command Chinese Peoples Liberat Army, Lanzhou Gen Hosp, Dept Pathol, Lanzhou 730050, Gansu, Peoples R China
4.Lanzhou Univ, Sch Pharm, Lanzhou 730000, Gansu, Peoples R China
5.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式
GB/T 7714		 Tian, Ning,He, Jinpeng,Wang, Bing,et al. miR-185 enhances the inhibition of proliferation and migration induced by ionizing radiation in melanoma[J]. ONCOLOGY LETTERS,2017,13:2442-2448.
APA Tian, Ning.,He, Jinpeng.,Wang, Bing.,Li, He.,Lin, Sulan.,...&Yang, Yanli.(2017).miR-185 enhances the inhibition of proliferation and migration induced by ionizing radiation in melanoma.ONCOLOGY LETTERS,13,2442-2448.
MLA Tian, Ning,et al."miR-185 enhances the inhibition of proliferation and migration induced by ionizing radiation in melanoma".ONCOLOGY LETTERS 13(2017):2442-2448.

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来源：近代物理研究所

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