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Microgravity induces inhibition of osteoblastic differentiation and mineralization through abrogating primary cilia

文献类型：期刊论文

作者Shi, Wengui2,4; Wang, Jufang2; Ding, Nan2; Xian, Cory J.3; Ma, Huiping1; Chen, Keming1; Wei, Wenjun2,4; Gao, Yuhai1; Zhou, Jian1; He, Jinpeng2
刊名SCIENTIFIC REPORTS
出版日期2017-05-12
卷号7页码:12
ISSN号2045-2322
DOI10.1038/s41598-017-02049-9
英文摘要It is well documented that microgravity in space environment leads to bone loss in astronauts. These physiological changes have also been validated by human and animal studies and modeled in cell-based analogs. However, the underlying mechanisms are elusive. In the current study, we identified a novel phenomenon that primary cilia (key sensors and functioning organelles) of rat calvarial osteoblasts (ROBs) gradually shrank and disappeared almost completely after exposure to simulated microgravity generated by a random positioning machine (RPM). Along with the abrogation of primary cilia, the differentiation, maturation and mineralization of ROBs were inhibited. We also found that the disappearance of primary cilia was prevented by treating ROBs with cytochalasin D, but not with LiCl or dynein light chain Tctex-type 1 (Dynlt1) siRNA. The repression of the differentiation, maturation and mineralization of ROBs was effectively offset by cytochalasin D treatment in microgravity conditions. Blocking ciliogenesis using intraflagellar transport protein 88 (IFT88) siRNA knockdown inhibited the ability of cytochalasin D to counteract this reduction of osteogenesis. These results indicate that the abrogation of primary cilia may be responsible for the microgravity's inhibition on osteogenesis. Reconstruction of primary cilia may become a potential strategy against bone loss induced by microgravity.
WOS关键词MESENCHYMAL STEM-CELLS ; RANDOM POSITIONING MACHINE ; OSTEOGENIC DIFFERENTIATION ; BONE-FORMATION ; FLUID SHEAR ; BED REST ; LENGTH ; CILIOGENESIS ; EXPRESSION ; MATURATION
资助项目International Science & Technology Cooperation Program of China[2015DFR30940] ; National Natural Sciences Foundation of China[81270963] ; National Natural Sciences Foundation of China[81471090] ; NHMRC[1042105]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000401262400059
出版者NATURE PUBLISHING GROUP
资助机构International Science & Technology Cooperation Program of China ; National Natural Sciences Foundation of China ; NHMRC
源URL[http://119.78.100.186/handle/113462/44782]  
专题近代物理研究所_生物物理研究室
通讯作者Wang, Jufang; Chen, Keming
作者单位1.Lanzhou Command CPLA, Lanzhou Gen Hosp, Inst Orthopaed, Lanzhou 730050, Peoples R China
2.Chinese Acad Sci, Inst Modern Phys, Gansu Key Lab Space Radiobiol, Lanzhou 730000, Peoples R China
3.Univ South Australia, Sch Pharm & Med Sci, Sansom Inst Hlth Res, Adelaide, SA 5001, Australia
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Shi, Wengui,Wang, Jufang,Ding, Nan,et al. Microgravity induces inhibition of osteoblastic differentiation and mineralization through abrogating primary cilia[J]. SCIENTIFIC REPORTS,2017,7:12.
APA Shi, Wengui.,Wang, Jufang.,Ding, Nan.,Xian, Cory J..,Ma, Huiping.,...&Xie, Yanfang.(2017).Microgravity induces inhibition of osteoblastic differentiation and mineralization through abrogating primary cilia.SCIENTIFIC REPORTS,7,12.
MLA Shi, Wengui,et al."Microgravity induces inhibition of osteoblastic differentiation and mineralization through abrogating primary cilia".SCIENTIFIC REPORTS 7(2017):12.

入库方式： OAI收割

来源：近代物理研究所

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