Changes in transcript levels of starch hydrolysis genes and raising citric acid production via carbon ion irradiation mutagenesis of Aspergillus niger
文献类型：期刊论文
| 作者 | Li, Wenjian2 ; Hu, Wei2; Chen, Jihong2; Wang, Shuyang2; Liu, Jing2; Chen, Hao1
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| 刊名 | PLOS ONE
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| 出版日期 | 2017-06-26 |
| 卷号 | 12页码:13 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0180120 |
| 英文摘要 | The filamentous ascomycete Aspergillus niger is well known for its ability to accumulate citric acid for the hydrolysis of starchy materials. To improve citric acid productivity, heavy ion beam mutagenesis was utilized to produce mutant A. niger strains with enhanced production of citric acid in this work. It was demonstrated that a mutant HW2 with high concentration of citric acid was isolated after carbon ion irradiation with the energy of 80Mev/mu, which was obvious increase higher than the original strain from liquefied corn starch as a feedstock. More importantly, with the evidence from the expression profiles of key genes and enzyme activity involved in the starch hydrolysis process between original strain and various phenotype mutants, our results confirmed that different transcript levels of key genes involving in starch hydrolysis process between original strain and mutants could be a significant contributor to different citric acid concentration in A. niger, such as, amyR and glaA, which therefore opened a new avenue for constructing genetically engineered A. niger mutants for high-yield citric acid accumulation in the future. As such, this work demonstrated that heavy ion beam mutagenesis presented an efficient alternative strategy to be developed to generate various phenotype microbe species mutants for functional genes research. |
| WOS关键词 | ARABIDOPSIS-THALIANA ; BEAM IRRADIATION ; MUTANT STRAIN ; AMYLASE GENES ; AMYR ; OPTIMIZATION ; ACTIVATOR ; RICE ; ACCUMULATION ; BIOREACTOR |
| 资助项目 | National Natural Science Foundation of China[11605259] ; western talents program of the Chinese Academy of Sciences[Y706030XB0] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000404537300040 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 资助机构 | National Natural Science Foundation of China ; western talents program of the Chinese Academy of Sciences |
| 源URL | [http://119.78.100.186/handle/113462/45393]  |
| 专题 | 近代物理研究所_生物物理研究室 |
| 通讯作者 | Hu, Wei; Chen, Jihong |
| 作者单位 | 1.Gansu Agr Univ, Coll Food Sci & Engn, Lanzhou, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wenjian,Hu, Wei,Chen, Jihong,et al. Changes in transcript levels of starch hydrolysis genes and raising citric acid production via carbon ion irradiation mutagenesis of Aspergillus niger[J]. PLOS ONE,2017,12:13. |
| APA | Li, Wenjian,Hu, Wei,Chen, Jihong,Wang, Shuyang,Liu, Jing,&Chen, Hao.(2017).Changes in transcript levels of starch hydrolysis genes and raising citric acid production via carbon ion irradiation mutagenesis of Aspergillus niger.PLOS ONE,12,13. |
| MLA | Li, Wenjian,et al."Changes in transcript levels of starch hydrolysis genes and raising citric acid production via carbon ion irradiation mutagenesis of Aspergillus niger".PLOS ONE 12(2017):13. |
入库方式： OAI收割
来源：近代物理研究所
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