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miR-300 regulates cellular radiosensitivity through targeting p53 and apaf1 in human lung cancer cells

文献类型：期刊论文

作者Cai, Hui5,6; Wei, Wenjun2,3,7; Lu, Zhiwei1; Wei, Li5,6; Hua, Junrui2,3; Feng, Xiu2,3,4; He, Jinpeng2,3; Ding, Nan2,3; Li, He2,3,7; Zhang, Yanan2,3
刊名CELL CYCLE
出版日期2017
卷号16页码:1943-1953
关键词apoptosis ionizing radiation lung cancer miR-300 senescence
ISSN号1538-4101
DOI10.1080/15384101.2017.1367070
英文摘要microRNAs (miRNAs) play a crucial role in mediation of the cellular sensitivity to ionizing radiation (IR). Previous studies revealed that miR-300 was involved in the cellular response to IR or chemotherapy drug. However, whether miR-300 could regulate the DNA damage responses induced by extrinsic genotoxic stress in human lung cancer and the underlying mechanism remain unknown. In this study, the expression of miR-300 was examined in lung cancer cells treated with IR, and the effects of miR-300 on DNA damage repair, cell cycle arrest, apoptosis and senescence induced by IR were investigated. It was found that IR induced upregulation of endogenous miR-300, and ectopic expression of miR-300 by transfected with miR-300 mimics not only greatly enhanced the cellular DNA damage repair ability but also substantially abrogated the G2 cell cycle arrest and apoptosis induced by IR. Bioinformatic analysis predicted that p53 and apaf1 were potential targets of miR-300, and the luciferase reporter assay showed that miR-300 significantly suppressed the luciferase activity through binding to the 3-UTR of p53 or apaf1 mRNA. In addition, overexpression of miR-300 significantly reduced p53/apaf1 and/or IR-induced p53/apaf1 protein expression levels. Flow cytomertry analysis and colony formation assay showed that miR-300 desensitized lung cancer cells to IR by suppressing p53-dependent G2 cell cycle arrest, apoptosis and senescence. These data demonstrate that miR-300 regulates the cellular sensitivity to IR through targeting p53 and apaf1 in lung cancer cells.
WOS关键词DNA-DAMAGE CHECKPOINT ; IONIZING-RADIATION ; TRANSCRIPTIONAL TARGET ; GASTRIC-CANCER ; G(2) ARREST ; MICRORNAS ; MUTATIONS ; GENE ; RADIOTHERAPY ; MODULATION
资助项目National Nature Science Foundation of China[11405232] ; National Nature Science Foundation of China[31270895] ; National Nature Science Foundation of China[31400723] ; Science and Technology Research Project of Gansu Province[145RTSA012] ; Natural Science Foundation of Gansu Province[145RJZA117]
WOS研究方向Cell Biology
语种英语
WOS记录号WOS:000414094300015
出版者TAYLOR & FRANCIS INC
资助机构National Nature Science Foundation of China ; Science and Technology Research Project of Gansu Province ; Natural Science Foundation of Gansu Province
源URL[http://119.78.100.186/handle/113462/45937]  
专题近代物理研究所_生物物理研究室
通讯作者Wang, Jufang
作者单位1.Gansu Univ Chinese Med, Major Dis Prevent & Control Mol Med & Tradit Chin, Lanzhou, Gansu, Peoples R China
2.Chinese Acad Sci, Inst Modern Phys, Key Lab Space Radiobiol Gansu Prov, Lanzhou, Gansu, Peoples R China
3.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Gansu, Peoples R China
4.Lanzhou Univ, Sch Pharm, Lanzhou, Gansu, Peoples R China
5.Gansu Prov Hosp, Clin Lab, Lanzhou, Gansu, Peoples R China
6.Gansu Prov Hosp, Gen Surg Dept, Lanzhou, Gansu, Peoples R China
7.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Cai, Hui,Wei, Wenjun,Lu, Zhiwei,et al. miR-300 regulates cellular radiosensitivity through targeting p53 and apaf1 in human lung cancer cells[J]. CELL CYCLE,2017,16:1943-1953.
APA Cai, Hui.,Wei, Wenjun.,Lu, Zhiwei.,Wei, Li.,Hua, Junrui.,...&Wang, Bing.(2017).miR-300 regulates cellular radiosensitivity through targeting p53 and apaf1 in human lung cancer cells.CELL CYCLE,16,1943-1953.
MLA Cai, Hui,et al."miR-300 regulates cellular radiosensitivity through targeting p53 and apaf1 in human lung cancer cells".CELL CYCLE 16(2017):1943-1953.

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来源：近代物理研究所

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