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Identification of Substitutions and Small Insertion-Deletions Induced by Carbon-Ion Beam Irradiation in Arabidopsis thaliana

文献类型：期刊论文

作者Guo, Tao2; Shu, Qingyao3; Luo, Wenlong2; Zhou, Libin5; Du, Yan5; Luo, Shanwei4,5; Li, Xin5; Yang, Jiangyan5; Cui, Tao4,5; Li, Wenjian5
刊名FRONTIERS IN PLANT SCIENCE
出版日期2017-10-27
卷号8页码:15
关键词Arabidopsis thaliana carbon-ion beam (CIB) irradiation mutation molecular spectrum small INDELs substitutions whole genome-wide re-sequencing
ISSN号1664-462X
DOI10.3389/fpls.2017.01851
英文摘要Heavy-ion beam irradiation is one of the principal methods used to create mutants in plants. Research on mutagenic effects and molecular mechanisms of radiation is an important subject that is multi-disciplinary. Here, we re-sequenced 11 mutagenesis progeny (M3) Arabidopsis thaliana lines derived from carbon-ion beam (CIB) irradiation, and subsequently focused on substitutions and small insertion-deletion (INDELs). We found that CIB inducedmore substitutions (320) than INDELs (124). Meanwhile, the single base INDELs weremore prevalent than those in large size (>= 2 bp). In details, the detected substitutions showed an obvious bias of C > T transitions, by activating the formation of covalent linkages between neighboring pyrimidine residues in the DNA sequence. An A and T bias was observed among the single base INDELs, in which most of these were induced by replication slippage at either the homopolymer or polynucleotide repeat regions. The mutation rate of 200-Gy CIB irradiation was estimated as 3.37 x 10(-7) per site. Different from previous researches which mainly focused on the phenotype, chromosome aberration, genetic polymorphism, or sequencing analysis of specific genes only, our study revealed genome-wide molecular profile and rate of mutations induced by CIB irradiation. We hope our data could provide valuable clues for explaining the potential mechanism of plant mutation breeding by CIB irradiation.
WOS关键词FAST-NEUTRON IRRADIATION ; CLUSTERED DNA-DAMAGE ; HIGH-LET IRRADIATION ; MOLECULAR NATURE ; MUTATIONS ; MUTANT ; RADIATION ; GENES ; PLANTS ; GAMMA
资助项目National Key Research and Development Program[2016YFD0102106] ; Chinese Academy of Sciences (CAS)[XDA08040111] ; National Natural Science Foundation of China[11205218] ; National Natural Science Foundation of China[11275171] ; National Natural Science Foundation of China[U1432126] ; Light of West China Program[Y506020] ; Light of West China Program[Y606020] ; Youth Innovation Promotion Association of CAS[2015337]
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000413815200001
出版者FRONTIERS MEDIA SA
资助机构National Key Research and Development Program ; Chinese Academy of Sciences (CAS) ; National Natural Science Foundation of China ; Light of West China Program ; Youth Innovation Promotion Association of CAS
源URL[http://119.78.100.186/handle/113462/45972]  
专题近代物理研究所_生物物理研究室
通讯作者Zhou, Libin
作者单位1.Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou, Gansu, Peoples R China
2.South China Agr Univ, Natl Engn Res Ctr Plant Space Breeding, Guangzhou, Guangdong, Peoples R China
3.Zhejiang Univ, Natl Key Lab Rice Biol, Inst Crop Sci, Hangzhou, Zhejiang, Peoples R China
4.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
5.Chinese Acad Sci, Inst Modern Phys, Biophys Grp, Lanzhou, Gansu, Peoples R China
推荐引用方式
GB/T 7714		 Guo, Tao,Shu, Qingyao,Luo, Wenlong,et al. Identification of Substitutions and Small Insertion-Deletions Induced by Carbon-Ion Beam Irradiation in Arabidopsis thaliana[J]. FRONTIERS IN PLANT SCIENCE,2017,8:15.
APA Guo, Tao.,Shu, Qingyao.,Luo, Wenlong.,Zhou, Libin.,Du, Yan.,...&Chen, Xia.(2017).Identification of Substitutions and Small Insertion-Deletions Induced by Carbon-Ion Beam Irradiation in Arabidopsis thaliana.FRONTIERS IN PLANT SCIENCE,8,15.
MLA Guo, Tao,et al."Identification of Substitutions and Small Insertion-Deletions Induced by Carbon-Ion Beam Irradiation in Arabidopsis thaliana".FRONTIERS IN PLANT SCIENCE 8(2017):15.

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来源：近代物理研究所

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