Tumor Cell-Accelerated Senescence Is Associated With DNA-PKcs Status and Telomere Dysfunction Induced by Radiation
文献类型：期刊论文
| 作者 | Lu, Dong1 ; Guo, Xiaopeng1,2; Zhang, Miaomiao1,2; Li, Wenjian1; Gao, Yue1,2
|
| 刊名 | DOSE-RESPONSE
 |
| 出版日期 | 2018-04-23 |
| 卷号 | 16期号:9页码:569 |
| 关键词 | accelerated senescence DNA-PKcs telomere dysfunction radiation |
| ISSN号 | 1559-3258 |
| DOI | 10.1177/1559325818771527 |
| 英文摘要 | Whether telomere structure integrity is related to radiosensitivity is not well investigated thus far. In this study, we investigated the relation between telomere instability and radiation-induced accelerated senescence. Partial knockdown of DNA-dependent catalytic subunit of protein kinase (DNA-PKcs) in human breast cancer cell line MCF-7 was established by small interfering RNA. Radiosensitivity of control and DNA-PKcs knockdown MCF-7 cells was analyzed by clonogenetic assay. Cell growth was measured by real-time cell electronic sensing. Senescence and apoptosis were evaluated by beta-galactosidase histochemical staining and fluorescence-activated cell sorting, respectively. DNA damage was determined by long polymerase chain reaction (PCR). Telomere length and integrity were analyzed by real-time PCR and cytogenetic assay, respectively. DNA-PKcs knockdown MCF-7 cells were more sensitive to X-irradiation than control cells. Further investigation revealed that accelerated senescence is more pronounced than apoptosis in cells after radiation, particularly in DNA-PKcs knockdown cells. The cytogenetic assay and kinetics of DNA damage repair revealed that the role of telomere end-capping in DNA-PKcs, rather than DNA damage repair, was more relevant to radiosensitivity. To our knowledge, this is the first study to show that DNA-PKcs plays an important role in radiation-induced accelerated senescence via maintenance of telomere integrity in MCF-7 cells. These results could be useful for future understanding of the radiation-induced genome instability and its consequences. |
| WOS关键词 | STRAND BREAK REPAIR ; IONIZING-RADIATION ; GROWTH ARREST ; DEFICIENCY ; APOPTOSIS ; RADIOSENSITIVITY ; CHROMOSOMES ; FIBROBLASTS ; EXPRESSION ; STRESS |
| 资助项目 | Chinese Academy of Sciences[CAS-ITRI 201705] ; Industrial Technology Research Institute[CAS-ITRI 201705] |
| WOS研究方向 | Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000432056700001 |
| 出版者 | SAGE PUBLICATIONS INC |
| 资助机构 | Chinese Academy of Sciences ; Industrial Technology Research Institute |
| 源URL | [http://119.78.100.186/handle/113462/47717]  |
| 专题 | 近代物理研究所_生物物理研究室 |
| 通讯作者 | Lu, Dong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Dong,Guo, Xiaopeng,Zhang, Miaomiao,et al. Tumor Cell-Accelerated Senescence Is Associated With DNA-PKcs Status and Telomere Dysfunction Induced by Radiation[J]. DOSE-RESPONSE,2018,16(9):569. |
| APA | Lu, Dong,Guo, Xiaopeng,Zhang, Miaomiao,Li, Wenjian,&Gao, Yue.(2018).Tumor Cell-Accelerated Senescence Is Associated With DNA-PKcs Status and Telomere Dysfunction Induced by Radiation.DOSE-RESPONSE,16(9),569. |
| MLA | Lu, Dong,et al."Tumor Cell-Accelerated Senescence Is Associated With DNA-PKcs Status and Telomere Dysfunction Induced by Radiation".DOSE-RESPONSE 16.9(2018):569. |
入库方式： OAI收割
来源：近代物理研究所
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