miR-3928 activates ATR pathway by targeting Dicer
文献类型：期刊论文
| 作者 | Wu, Xin1,2; Yao, Bin1,2; Ye, Caiyong1,2; Hu, Wentao1,2; Chang, Lei1,2; Zhou, Guangming1 ; Wang, Jufang1; Ding, Nan1,2; Song, Lei1,2
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| 刊名 | RNA BIOLOGY
 |
| 出版日期 | 2012-10-01 |
| 卷号 | 9页码:1247-1254 |
| 关键词 | Microrna Dicer Dna Damage G1 Arrest Ionizing Radiation |
| ISSN号 | 1547-6286 |
| DOI | 10.4161/rna.21821 |
| 文献子类 | Article |
| 英文摘要 | Alterations in microRNA (miRNA) expression have been observed in cells subjected to exogenous stresses, implying that miRNAs play an important role in cellular stress response; however, the underlying mechanism is still largely unknown. In the present study, we found that miR-3928 was implicated in cellular response to ionizing radiation. After exposed to X-rays, miR-3928 expression increased in 1.5 h and then decreased, meanwhile Dicer, a key component in the miRNA processing machinery, increased gradually. An oscillation was observed in the expression of both mature miR-3928 and Dicer mRNA from 2 h to 3.5 h in irradiated cells. Then, we verified that miR-3928 directly bound to the 3'-untranslated region of Dicer mRNA. Consequently, Dicer expression was suppressed and the maturation of other miRNAs including miR-185, miR-300, and miR-663, was inhibited. Overexpression of miR-3928 induced DNA damage, activated ATR, and phosphorylated Chk1 accompanied by G1 arrest. Taken together, these findings replenished ATR/Chk1 pathway by revealing a novel miRNA regulatory network in response to exogenous stress, in which miR-3928 plays an important role in regulating the expression of Dicer. |
| WOS关键词 | DNA-DAMAGE RESPONSE ; HUMAN ENDOTHELIAL-CELLS ; CELLULAR STRESS ; CANCER-CELLS ; SMALL RNAS ; EXPRESSION ; APOPTOSIS ; SURVIVAL ; MIRNAS |
| 资助项目 | National Natural Science Foundation of China[10979062] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000310575700006 |
| 出版者 | LANDES BIOSCIENCE |
| 源URL | [http://119.78.100.186/handle/113462/47792]  |
| 专题 | 近代物理研究所_生物物理研究室 |
| 通讯作者 | Wang, Jufang |
| 作者单位 | 1.Chinese Acad Sci, Dept Space Radiobiol, Key Lab Heavy Ion Radiat Biol & Med, Inst Modern Phys, Lanzhou, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Xin,Yao, Bin,Ye, Caiyong,et al. miR-3928 activates ATR pathway by targeting Dicer[J]. RNA BIOLOGY,2012,9:1247-1254. |
| APA | Wu, Xin.,Yao, Bin.,Ye, Caiyong.,Hu, Wentao.,Chang, Lei.,...&Song, Lei.(2012).miR-3928 activates ATR pathway by targeting Dicer.RNA BIOLOGY,9,1247-1254. |
| MLA | Wu, Xin,et al."miR-3928 activates ATR pathway by targeting Dicer".RNA BIOLOGY 9(2012):1247-1254. |
入库方式： OAI收割
来源：近代物理研究所
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