Effects of X-ray and carbon ion beam irradiation on membrane permeability and integrity in Saccharomyces cerevisiae cells
文献类型：期刊论文
| 作者 | Miao, Jianshun1,3; Xia, Jiefang1,3; Lu, Dong1,3; Cao, Guozhen1,2,3; Zhang, Miaomiao1,3 ; Wang, Jufang1,3; Li, Wenjian1,3
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| 刊名 | JOURNAL OF RADIATION RESEARCH
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| 出版日期 | 2015-03-01 |
| 卷号 | 56页码:294-304 |
| 关键词 | Cell Membrane Permeability X-ray Carbon Ion Flow Cytometry Saccharomyces Cerevisiae |
| ISSN号 | 0449-3060 |
| DOI | 10.1093/jrr/rru114 |
| 文献子类 | Article |
| 英文摘要 | Saccharomyces cerevisiae has served as a eukaryotic model in radiation biology studies of cellular responses to ionizing radiation (IR). Research in this field has thus far mainly been focused on DNA strand breaks, DNA base damage, or inhibition of protein activity. However, the effects of IR on S. cerevisiae cell membranes have barely been studied. Here, we investigated the changes in the permeability and integrity of S. cerevisiae cell membranes induced by high-linear energy transfer carbon ion (CI) beam or low-linear energy transfer X-ray. After CI exposure, protein elution and nucleotide diffusion were more pronounced than after X-ray treatment at the same doses, although these features were most prevalent following irradiation doses of 25-175 Gy. Flow cytometry of forward scatter light versus side scatter light and double-staining with fluorescein diacetate and propidium iodide showed that CI and X-ray irradiation significantly affected S. cerevisiae cell membrane integrity and cellular enzyme activity compared with untreated control cells. The extent of lesions in CI-irradiated cells, which exhibited markedly altered morphology and size, was greater than that in X-ray-irradiated cells. The relationships between permeabilized cells, esterase activity, and non-viable cell numbers furthermore indicated that irradiation-induced increases in cell permeabilization and decreases in esterase activity are dependent on the type of radiation and that these parameters correspond well with cell viability. These results also indicate that the patterns of cell inactivity due to X-ray or CI irradiation may be similar in terms of cell membrane damage. |
| WOS关键词 | DOUBLE-STRAND BREAKS ; HIGH-LET IRRADIATION ; LACTOCOCCUS-LACTIS ; FLUORESCENCE ASSESSMENT ; MICROWAVE IRRADIATION ; FLOW-CYTOMETRY ; DAMAGE ; YEAST ; VIABILITY ; RADIATION |
| 资助项目 | National Natural Science Foundation of China[U1232126] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000352490200010 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://119.78.100.186/handle/113462/47931]  |
| 专题 | 近代物理研究所_生物物理研究室 |
| 通讯作者 | Lu, Dong |
| 作者单位 | 1.Key Lab Microbial Resources Exploit & Applicat, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Sch Pharm, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Miao, Jianshun,Xia, Jiefang,Lu, Dong,et al. Effects of X-ray and carbon ion beam irradiation on membrane permeability and integrity in Saccharomyces cerevisiae cells[J]. JOURNAL OF RADIATION RESEARCH,2015,56:294-304. |
| APA | Miao, Jianshun.,Xia, Jiefang.,Lu, Dong.,Cao, Guozhen.,Zhang, Miaomiao.,...&Li, Wenjian.(2015).Effects of X-ray and carbon ion beam irradiation on membrane permeability and integrity in Saccharomyces cerevisiae cells.JOURNAL OF RADIATION RESEARCH,56,294-304. |
| MLA | Miao, Jianshun,et al."Effects of X-ray and carbon ion beam irradiation on membrane permeability and integrity in Saccharomyces cerevisiae cells".JOURNAL OF RADIATION RESEARCH 56(2015):294-304. |
入库方式： OAI收割
来源：近代物理研究所
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