Reference Gene Selection for Quantitative Real-Time PCR Normalization in Reaumuria soongorica
文献类型：期刊论文
| 作者 | Dong, Xicun4 ; Yan, Xia2,3; Ma, Xiao-Fei2; Chen, Peng1; Xiao, Honglang3; Yin, Hengxia2; Zhang, Wen2
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| 刊名 | PLOS ONE
 |
| 出版日期 | 2014-08-12 |
| 卷号 | 9 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0104124 |
| 文献子类 | Article |
| 英文摘要 | Despite its superiority for evaluating gene expression, real-time quantitative polymerase chain reaction (qPCR) results can be significantly biased by the use of inappropriate reference genes under different experimental conditions. Reaumuria soongorica is a dominant species of desert ecosystems in arid central Asia. Given the increasing interest in ecological engineering and potential genetic resources for arid agronomy, it is important to analyze gene function. However, systematic evaluation of stable reference genes should be performed prior to such analyses. In this study, the stabilities of 10 candidate reference genes were analyzed under 4 kinds of abiotic stresses (drought, salt, dark, and heat) within 4 accessions (HG010, HG020, XGG030, and XGG040) from 2 different habitats using 3 algorithms (geNorm, NormFinder, and BestKeeper). After validation of the ribulose-1,5-bisphosphate carboxylase/oxygenase large unite (rbcL) expression pattern, our data suggested that histone H2A (H2A) and eukaryotic initiation factor 4A-2 (EIF4A2) were the most stable reference genes, cyclophilin (CYCL) was moderate, and elongation factor 1 alpha (EF1 alpha) was the worst choice. This first systematic analysis for stably expressed genes will facilitate future functional analyses and deep mining of genetic resources in R. soongorica and other species of the Reaumuria genus. |
| WOS关键词 | POLYMERASE-CHAIN-REACTION ; RT-PCR ; INTERNAL CONTROL ; HOUSEKEEPING GENES ; ABIOTIC STRESS ; DROUGHT STRESS ; EXPRESSION ; VALIDATION ; IDENTIFICATION ; ARABIDOPSIS |
| 资助项目 | National Natural Science Foundation of China[91125025] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000341230400033 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://119.78.100.186/handle/113462/49213]  |
| 专题 | 近代物理研究所_生物物理研究室 |
| 通讯作者 | Ma, Xiao-Fei |
| 作者单位 | 1.Huazhong Agr Univ, Fac Plant Sci & Technol, Wuhan, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Key Lab Stress Physiol & Ecol Cold & Arid Reg Gan, Lanzhou, Peoples R China 3.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Key Lab Inland River Ecohydrol, Lanzhou, Peoples R China 4.Chinese Acad Sci, Inst Modern Phys, Dept Radiobiol, Lanzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Xicun,Yan, Xia,Ma, Xiao-Fei,et al. Reference Gene Selection for Quantitative Real-Time PCR Normalization in Reaumuria soongorica[J]. PLOS ONE,2014,9. |
| APA | Dong, Xicun.,Yan, Xia.,Ma, Xiao-Fei.,Chen, Peng.,Xiao, Honglang.,...&Zhang, Wen.(2014).Reference Gene Selection for Quantitative Real-Time PCR Normalization in Reaumuria soongorica.PLOS ONE,9. |
| MLA | Dong, Xicun,et al."Reference Gene Selection for Quantitative Real-Time PCR Normalization in Reaumuria soongorica".PLOS ONE 9(2014). |
入库方式： OAI收割
来源：近代物理研究所
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