MiR-21 is involved in radiation-induced bystander effects
文献类型：期刊论文
| 作者 | Xu, Shuai1,2; Ding, Nan1 ; Pei, Hailong1,2; Hu, Wentao1; Wei, Wenjun1,2; Zhang, Xurui1,2; Zhou, Guangming1; Wang, Jufang1
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| 刊名 | RNA BIOLOGY
 |
| 出版日期 | 2014-09-01 |
| 卷号 | 11页码:1161-1170 |
| ISSN号 | 1547-6286 |
| 关键词 | Bystander Effect Microrna Mir-21 Medium Radiation Ir Ionizing Radiation Ribe Radiation-induced Bystander Effects Ros Reactive Oxygen Species Tnf Tumor Necrosis Factor-alpha Tgf1 Transforming Growth Factor-beta 1 3-utr 3-untranslated Region Mirna Microrna Mir-21 Microrna-21 Pre-mir-21 The Precursor Of Mir-21 |
| DOI | 10.4161/rna.34380 |
| 文献子类 | Article |
| 英文摘要 | Radiation-induced bystander effects are well-established phenomena, in which DNA damage responses are induced not only in the directly irradiated cells but also in the non-irradiated bystander cells through intercellular signal transmission. Recent studies hint that bystander effects are possibly mediated via small non-coding RNAs, especially microRNAs. Thus, more details about the roles of microRNA in bystander effects are urgently needed to be elucidated. Here we demonstrated that bystander effects were induced in human fetal lung MRC-5 fibroblasts through medium-mediated way by different types of radiation. We identified a set of differentially expressed microRNAs in the cell culture medium after irradiation, among which the up-regulation of miR-21 was further verified with qRT-PCR. In addition, we found significant upregulation of miR-21 in both directly irradiated cells and bystander cells, which was confirmed by the expression of miR-21 precursor and its target genes. Transfection of miR-21 mimics into non-irradiated MRC-5 cells caused bystander-like effects. Taken together, our data reveals that miR-21 is involved in radiation-induced bystander effects. Elucidation of such a miRNA-mediated bystander effect is of utmost importance in understanding the biological processes related to ionizing radiation and cell-to-cell communication. |
| WOS关键词 | INDUCED GENOMIC INSTABILITY ; IONIZING-RADIATION ; HUMAN FIBROBLASTS ; CIRCULATING MICRORNAS ; CLONOGENIC SURVIVAL ; UNIRRADIATED CELLS ; GLIOMA-CELLS ; NITRIC-OXIDE ; IN-VIVO ; CANCER |
| 资助项目 | National Natural Science Foundation of China[31270895] ; National Natural Science Foundation of China[U1232125] ; National Natural Science Foundation of China[11335011] ; National Natural Science Foundation of China[31370846] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | LANDES BIOSCIENCE |
| WOS记录号 | WOS:000348463500008 |
| 源URL | [http://119.78.100.186/handle/113462/49317]  |
| 专题 | 近代物理研究所_生物物理研究室 |
| 通讯作者 | Wang, Jufang |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Dept Space Radiobiol, Lanzhou, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Shuai,Ding, Nan,Pei, Hailong,et al. MiR-21 is involved in radiation-induced bystander effects[J]. RNA BIOLOGY,2014,11:1161-1170. |
| APA | Xu, Shuai.,Ding, Nan.,Pei, Hailong.,Hu, Wentao.,Wei, Wenjun.,...&Wang, Jufang.(2014).MiR-21 is involved in radiation-induced bystander effects.RNA BIOLOGY,11,1161-1170. |
| MLA | Xu, Shuai,et al."MiR-21 is involved in radiation-induced bystander effects".RNA BIOLOGY 11(2014):1161-1170. |
入库方式： OAI收割
来源：近代物理研究所
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