Assessment of mouse BMMNC DNA damage with a two-tailed comet assay after X-ray and carbon ion total body irradiation
文献类型：期刊论文
| 作者 | Wang, Zhuan-Zi1 ; Li, Wen-Jian1; Wei, Wei1; Dang, Bing-Rong1; Liu, Fang1,2
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| 刊名 | NUCLEAR SCIENCE AND TECHNIQUES
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| 出版日期 | 2018-06 |
| 卷号 | 29页码:77 |
| 关键词 | Two-tailed comet assay, carbon ion irradiation X-ray irradiation Bone marrow mononuclear cells DNA damage |
| ISSN号 | 1001-8042 |
| DOI | 10.1007/s41365-018-0421-1 |
| 英文摘要 | The two-tailed comet assay (2T-comet assay) is a method for simultaneously evaluating DNA single-strand breaks (SSBs) and double-strand breaks (DSBs). In the present study, the endonuclease DNase I and hydrogen peroxide were used to induce DSBs and SSBs in bone marrow mononuclear cells (BMMNCs) from mice, and the damaged DNAs were assessed with a 2T-comet assay. The results demonstrated that this method can detect and discriminate between BMMNC DNA SSBs and DSBs simultaneously. Using this method, we studied DNA damage in BMMNCs from female BALB/c mice after total body irradiation with X-rays and carbon ions. The results indicated that these two types of radiation induced serious DNA damage in BMMNCs in a dose-dependent manner. The DNA damage induced by carbon ions was more severe than that induced by X-rays at the same dose, and a high dose of carbon ion radiation was more likely to cause death in mice. The DSBs and SSBs induced by X-rays were the highest on the 3rd day post-IR. For carbon ion radiation, DSBs were the most serious on the 3rd day, while SSBs were more obvious on the 3rd day and 13th day post-IR. The ratio of DSBs/SSBs was clearly related to the different types of radiation. |
| WOS关键词 | DOUBLE-STRAND BREAKS ; OXIDATIVE STRESS ; BONE-MARROW ; IONIZING-RADIATION ; FE-56 IONS ; STEM-CELLS ; REPAIR ; INJURY ; MICE ; SENESCENCE |
| 资助项目 | National Natural Science Foundation of China[11575259] |
| WOS研究方向 | Nuclear Science & Technology ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000433263900001 |
| 出版者 | SPRINGER SINGAPORE PTE LTD |
| 源URL | [http://119.78.100.186/handle/113462/56833]  |
| 专题 | 近代物理研究所_生物物理研究室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Zhuan-Zi,Li, Wen-Jian,Wei, Wei,et al. Assessment of mouse BMMNC DNA damage with a two-tailed comet assay after X-ray and carbon ion total body irradiation[J]. NUCLEAR SCIENCE AND TECHNIQUES,2018,29:77. |
| APA | Wang, Zhuan-Zi,Li, Wen-Jian,Wei, Wei,Dang, Bing-Rong,&Liu, Fang.(2018).Assessment of mouse BMMNC DNA damage with a two-tailed comet assay after X-ray and carbon ion total body irradiation.NUCLEAR SCIENCE AND TECHNIQUES,29,77. |
| MLA | Wang, Zhuan-Zi,et al."Assessment of mouse BMMNC DNA damage with a two-tailed comet assay after X-ray and carbon ion total body irradiation".NUCLEAR SCIENCE AND TECHNIQUES 29(2018):77. |
入库方式： OAI收割
来源：近代物理研究所
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