中国科学院机构知识库网格系统: Variation in the phenotypic features and transcripts of thermo-sensitive leaf-color mutant induced by carbon ion beam in Green wandering jew (Tradescantia fluminensis)

Variation in the phenotypic features and transcripts of thermo-sensitive leaf-color mutant induced by carbon ion beam in Green wandering jew (Tradescantia fluminensis)

文献类型：期刊论文


作者	Liu, Ruiyuan1,3 ; Li, Wenjian3 ; Zhang, Feng 1,2; Qu, Ying3 ; Jin, Wenjie3 ; Yu, Lixia3 ; Gu, Wenting 3; Dong, Xicun3
刊名	SCIENTIA HORTICULTURAE
出版日期	2016-12-14
卷号	213 页码:303-313
关键词	Wandering jew Temperature Mutant Chlorophyll Anthocyanin
ISSN号	0304-4238
DOI	10.1016/j.scienta.2016.11.001
英文摘要	Leaf color mutants are ideal materials for studying physiological processes in plants. Here, a thermos sensitive leaf-color mutant of Green wandering jew was isolated after carbon ions irradiation, which was designated as mt. The color of young mutant leaves was more sensitive to variations of temperature, however, the young leaves of wild type remained green under low - temperature conditions (6 degrees C-20 degrees C). To elucidate the characteristics of pigmentation in mutant leaves under room temperature conditions (25 C) and low - temperature conditions (7 degrees C), the ultra-structural, pigment composition, molecular mechanisms and anthocyanin accumulation involved in this phenomenon have been investigated in four independent experiments. The results showed that chloroplasts of mutants exhibited abnormal morphology and distribution at 25 degrees C, and under low - temperature conditions(7 degrees C), the chloroplasts converted into leucoplast in leaves on mutants. Temperature change affected the rate of color transition, chlorophyll and anthocyanin concentrations in leaves on mutants. Molecular analysis indicated that all the anthocyanin biosynthetic genes and regulatory genes were constitutively up-regulated in mutant leaves at 7 C. The other anthocyanin biosynthesis and regulatory genes showed similar expression levels between mutant and wild type except PAL, CHS, ANS were up-regulated at 25 C. HPLC analysis of anthocyanins in mutant and wild type leaves revealed that the contents of cyanidin, petunidin and delphinidin were significantly lower than those of under low-temperature conditions (7 degrees C) except pelargonidin was not detected in mutant leaves under room - temperature conditions(25 degrees C). The HPLC profile also indicated the highest levels of flavonols in mutant leaves at 7 C significantly. Overall, above results indicated a close similarity among ultra-structures, pigment compositions, transcript amount and anthocyanin accumulation were tightly associated with temperature variation in leaves of leaf color mutant. These findings would provide better understanding of the mechanism of pigmentation changes in mutant leaves under different temperature conditions in Wandering jew. (C) 2016 Elsevier B.V. All rights reserved.
WOS关键词	BIOSYNTHESIS-RELATED GENES ; ANTHOCYANIN BIOSYNTHESIS ; CHLOROPLAST DEVELOPMENT ; ARABIDOPSIS-THALIANA ; IRRADIATION ; EXPRESSION ; STRESS ; ACCUMULATION ; L. ; MUTAGENESIS
资助项目	Western Light Co-scholar Program[29Y406020]
WOS研究方向	Agriculture
语种	英语
WOS记录号	WOS:000390502600037
出版者	ELSEVIER SCIENCE BV
源URL	[http://119.78.100.186/handle/113462/56845]
专题	近代物理研究所_生物物理研究室
通讯作者	Li, Wenjian; Zhang, Feng
作者单位	1.Gansu Agr Univ, Coll Agron, Lanzhou 730070, Peoples R China 2.Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Dept Biophys, 509 Nanchang Rd, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ruiyuan,Li, Wenjian,Zhang, Feng,et al. Variation in the phenotypic features and transcripts of thermo-sensitive leaf-color mutant induced by carbon ion beam in Green wandering jew (Tradescantia fluminensis)[J]. SCIENTIA HORTICULTURAE,2016,213:303-313.
APA	Liu, Ruiyuan.,Li, Wenjian.,Zhang, Feng.,Qu, Ying.,Jin, Wenjie.,...&Dong, Xicun.(2016).Variation in the phenotypic features and transcripts of thermo-sensitive leaf-color mutant induced by carbon ion beam in Green wandering jew (Tradescantia fluminensis).SCIENTIA HORTICULTURAE,213,303-313.
MLA	Liu, Ruiyuan,et al."Variation in the phenotypic features and transcripts of thermo-sensitive leaf-color mutant induced by carbon ion beam in Green wandering jew (Tradescantia fluminensis)".SCIENTIA HORTICULTURAE 213(2016):303-313.

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来源：近代物理研究所

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Variation in the phenotypic features and transcripts of thermo-sensitive leaf-color mutant induced by carbon ion beam in Green wandering jew (Tradescantia fluminensis)

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