中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Variation in the phenotypic features and transcripts of thermo-sensitive leaf-color mutant induced by carbon ion beam in Green wandering jew (Tradescantia fluminensis)

文献类型:期刊论文

作者Liu, Ruiyuan1,3; Li, Wenjian3; Zhang, Feng1,2; Qu, Ying3; Jin, Wenjie3; Yu, Lixia3; Gu, Wenting3; Dong, Xicun3
刊名SCIENTIA HORTICULTURAE
出版日期2016-12-14
卷号213页码:303-313
关键词Wandering jew Temperature Mutant Chlorophyll Anthocyanin
ISSN号0304-4238
DOI10.1016/j.scienta.2016.11.001
英文摘要Leaf color mutants are ideal materials for studying physiological processes in plants. Here, a thermos sensitive leaf-color mutant of Green wandering jew was isolated after carbon ions irradiation, which was designated as mt. The color of young mutant leaves was more sensitive to variations of temperature, however, the young leaves of wild type remained green under low - temperature conditions (6 degrees C-20 degrees C). To elucidate the characteristics of pigmentation in mutant leaves under room temperature conditions (25 C) and low - temperature conditions (7 degrees C), the ultra-structural, pigment composition, molecular mechanisms and anthocyanin accumulation involved in this phenomenon have been investigated in four independent experiments. The results showed that chloroplasts of mutants exhibited abnormal morphology and distribution at 25 degrees C, and under low - temperature conditions(7 degrees C), the chloroplasts converted into leucoplast in leaves on mutants. Temperature change affected the rate of color transition, chlorophyll and anthocyanin concentrations in leaves on mutants. Molecular analysis indicated that all the anthocyanin biosynthetic genes and regulatory genes were constitutively up-regulated in mutant leaves at 7 C. The other anthocyanin biosynthesis and regulatory genes showed similar expression levels between mutant and wild type except PAL, CHS, ANS were up-regulated at 25 C. HPLC analysis of anthocyanins in mutant and wild type leaves revealed that the contents of cyanidin, petunidin and delphinidin were significantly lower than those of under low-temperature conditions (7 degrees C) except pelargonidin was not detected in mutant leaves under room - temperature conditions(25 degrees C). The HPLC profile also indicated the highest levels of flavonols in mutant leaves at 7 C significantly. Overall, above results indicated a close similarity among ultra-structures, pigment compositions, transcript amount and anthocyanin accumulation were tightly associated with temperature variation in leaves of leaf color mutant. These findings would provide better understanding of the mechanism of pigmentation changes in mutant leaves under different temperature conditions in Wandering jew. (C) 2016 Elsevier B.V. All rights reserved.
WOS关键词BIOSYNTHESIS-RELATED GENES ; ANTHOCYANIN BIOSYNTHESIS ; CHLOROPLAST DEVELOPMENT ; ARABIDOPSIS-THALIANA ; IRRADIATION ; EXPRESSION ; STRESS ; ACCUMULATION ; L. ; MUTAGENESIS
资助项目Western Light Co-scholar Program[29Y406020]
WOS研究方向Agriculture
语种英语
WOS记录号WOS:000390502600037
出版者ELSEVIER SCIENCE BV
源URL[http://119.78.100.186/handle/113462/56845]  
专题近代物理研究所_生物物理研究室
通讯作者Li, Wenjian; Zhang, Feng
作者单位1.Gansu Agr Univ, Coll Agron, Lanzhou 730070, Peoples R China
2.Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Peoples R China
3.Chinese Acad Sci, Inst Modern Phys, Dept Biophys, 509 Nanchang Rd, Lanzhou 730000, Peoples R China
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GB/T 7714
Liu, Ruiyuan,Li, Wenjian,Zhang, Feng,et al. Variation in the phenotypic features and transcripts of thermo-sensitive leaf-color mutant induced by carbon ion beam in Green wandering jew (Tradescantia fluminensis)[J]. SCIENTIA HORTICULTURAE,2016,213:303-313.
APA Liu, Ruiyuan.,Li, Wenjian.,Zhang, Feng.,Qu, Ying.,Jin, Wenjie.,...&Dong, Xicun.(2016).Variation in the phenotypic features and transcripts of thermo-sensitive leaf-color mutant induced by carbon ion beam in Green wandering jew (Tradescantia fluminensis).SCIENTIA HORTICULTURAE,213,303-313.
MLA Liu, Ruiyuan,et al."Variation in the phenotypic features and transcripts of thermo-sensitive leaf-color mutant induced by carbon ion beam in Green wandering jew (Tradescantia fluminensis)".SCIENTIA HORTICULTURAE 213(2016):303-313.

入库方式: OAI收割

来源:近代物理研究所

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