The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation
文献类型：期刊论文
| 作者 | Wang, Shuyang ; Lu, Dong; Liu, Jing; Li, Wenjian; Wu, Qinghua; Chen, Jihong; Hu, Wei
|
| 刊名 | BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 60 |
| 关键词 | L. thermophilus mutant heavy ion irradiation L-(+)-lactic acid production carbon source fermentation characteristic |
| ISSN号 | 1516-8913 |
| DOI | 10.1590/1678-4324-2016160337 |
| 英文摘要 | Screening promising L. thermophiles with high productivity, high efficiency and strong adaptability are very important in lactic acid industry. For this purpose, 80MeV/u carbon ions were applied to irradiate L. thermophiles. After high-throughput screening, a mutant, named SRZ50, was obtained. Different carbon sources or nitrogen sources were provided to investigate carbon or nitrogen source utilization between mutant SRZ50 and wild type, and different fermentation periods were also chose to study fermentation characteristic between mutant SRZ50 and wild type. The results showed that mutant SRZ50 exhibited the enhanced L-(+)-lactic acid production from wild type. When glucose or fructose was the sole carbon source, the L(+)-lactic acid production by mutant SRZ50 was both the highest, respectively, 23.16 +/- 0.72 g/L or 23.24 +/- 0.66 g/L, which had a significant increase from that of wild type (P<0.01), following obvious increase in biomass (P<0.05). When yeast powder was the sole nitrogen source, it can promote mutant SRZ50 to accumulate the highest L-(+)-lactic acid accumulation, which also had a significant increase from that of wild type (P<0.01). Under different fermentation periods, it was obtained that mutant SRZ50 all exhibited significant increase in L-(+)-lactic acid accumulation from wild type. In conclusion, a mutant strain with improved production profiles for L-(+)-lactic acid, was obtained, indicating that heavy ions can be an efficient tool to improve metabolic product accumulations in microbes. |
| WOS关键词 | DIFFERENT NITROGEN-SOURCES ; LACTIC-ACID ; L(+)-LACTIC ACID ; RHIZOPUS-ORYZAE ; ARABIDOPSIS-THALIANA ; STRAIN IMPROVEMENT ; ASPERGILLUS-NIGER ; BEAM IRRADIATION ; CARBON-SOURCES ; CITRIC-ACID |
| 资助项目 | Agriculture Science Technology Achievement Transformation Fund[2013GB24910680] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000435656500001 |
| 出版者 | INST TECNOLOGIA PARANA |
| 源URL | [http://119.78.100.186/handle/113462/56850]  |
| 专题 | 近代物理研究所_生物物理研究室 |
| 通讯作者 | Wang, Shuyang |
| 作者单位 | Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Shuyang,Lu, Dong,Liu, Jing,et al. The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation[J]. BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY,2017,60. |
| APA | Wang, Shuyang.,Lu, Dong.,Liu, Jing.,Li, Wenjian.,Wu, Qinghua.,...&Hu, Wei.(2017).The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation.BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY,60. |
| MLA | Wang, Shuyang,et al."The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation".BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY 60(2017). |
入库方式： OAI收割
来源：近代物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。