RAPD analysis on avermectin high-producing strain of Streptomyces Avermilis Irradiated by C-12(6+) ions
文献类型：会议论文
| 作者 | Yarlagadda, P; Kim, YH; Zhang, XD; Ai, ZJ; Liang, Jianping2; Chen, Jihong2 ; Wang, Shuyang2; Bo, Yongheng1
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| 出版日期 | 2011 |
| 关键词 | C-12(6+) ions mutation Streptomyces avermitilis |
| 卷号 | 339 |
| DOI | 10.4028/www.scientific.net/AMR.339.701 |
| 页码 | 701-+ |
| 英文摘要 | Industrial Streptomyces avermitilis were irradiated by 80 MeV/u C-12(6+) ions whith were provided by the Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou Heavy Ion Accelerator. The high titers of mutants were obtained with different doses. The original strains and the high titers of mutants were detected and analyzed by Random Amplified Polymorphic DNA technology (RAPD). The results show that bands could be got from amplify the original strain by 4 primers of 28 primers and the high titers of mutants were amplified by the 4 primers. The amplified products were analysed by Jaccard formula. After 10 Gy, 30 Gy, 40 Gy, 45 Gy, 50 Gy, 70 Gy isodose irradiation, the similarity index were 0.63, 0.75, 0.71, 0.8, 0.61, 0.73. It was confirmed that at the DNA level, not only the high titers strains and the original mutant strain's DNA were different, but also the high titers of the different mutants' DNA were different. Therefore, ion implantation method changed the different Streptomyces avermitilis genetic material which promote the importance trace of avermectin biosynthesis changed, and different high titers of Streptomyces avermitilis were obtained. |
| 会议录 | ADVANCED MANUFACTURING SYSTEMS
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| 会议录出版者 | TRANS TECH PUBLICATIONS LTD |
| 会议录出版地 | KREUZSTRASSE 10, 8635 DURNTEN-ZURICH, SWITZERLAND |
| 语种 | 英语 |
| WOS研究方向 | Engineering ; Materials Science |
| WOS记录号 | WOS:000309383100136 |
| 源URL | [http://119.78.100.186/handle/113462/58566]  |
| 专题 | 近代物理研究所_生物物理研究室 |
| 通讯作者 | Bo, Yongheng |
| 作者单位 | 1.Gansu Agr Univ, Anim Med Coll, Lanzhou 730070, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yarlagadda, P,Kim, YH,Zhang, XD,et al. RAPD analysis on avermectin high-producing strain of Streptomyces Avermilis Irradiated by C-12(6+) ions[C]. 见:. |
入库方式： OAI收割
来源：近代物理研究所
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