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Enhanced production of L-lactic acid by Lactobacillus thermophilus SRZ50 mutant generated by high-linear energy transfer heavy ion mutagenesis

文献类型：期刊论文

作者Li, Wen-jian2; Hu, Wei2; Jiang, Ai-lian1,2; Chen, Ji-hong2; Lu, Dong2; Wang, Shu-yang2; Liu, Jing2; Tian, Xue-jiao1,2; Liu, Lu1,2
刊名ENGINEERING IN LIFE SCIENCES
出版日期2018-09-01
卷号18页码:626-634
关键词Heavy ion mutagenesis High productivity High-throughput screening Lactobacillus thermophilus L-lactic acid
ISSN号1618-0240
DOI10.1002/elsc.201800052
英文摘要The aim of this study was to improve L-lactic acid production of Lactobacillus thermophilus SRZ50. For this purpose, high efficient heavy-ion mutagenesis technique was performed using SRZ50 as the original strain. To enhance the screening efficiency for high yield L-lactic acid producers, a scale-down from shake flask to microtiter plate was developed. The results showed that 24-well U-bottom MTPs could well alternate shake flasks for L. thermophilus cultivation as a scale-down tool due to its a very good comparability to the shake flasks. Based on this microtiter plate screening method, two high L-lactic acid productivity mutants, A59 and A69, were successfully screened out, which presented, respectively, 15.8 and 16.2% higher productivities than that of the original strain. Based on fed-batch fermentation, the A69 mutant can accumulate 114.2 g/L L-lactic acid at 96 h. Hence, the proposed traditional microbial breeding method with efficient high-throughput screening assay was proved to be an appropriate strategy to obtain lactic acid-overproducing strain.
WOS关键词ROOM-TEMPERATURE PLASMA ; MICROBIAL-PRODUCTION ; STRAIN IMPROVEMENT ; FILAMENTOUS FUNGI ; MICROTITER PLATES ; D(-)-LACTIC ACID ; IRRADIATION ; FERMENTATION ; AVERMITILIS ; BEAMS
资助项目National Natural Science Foundation of China[11605259] ; National Natural Science Foundation of China[Y706030XB0]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000443557800001
出版者WILEY
资助机构National Natural Science Foundation of China
源URL[http://119.78.100.186/handle/113462/59410]  
专题近代物理研究所_生物物理研究室
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Chinese Acad Sci, Inst Modern Phys, Dept Biophys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式
GB/T 7714		 Li, Wen-jian,Hu, Wei,Jiang, Ai-lian,et al. Enhanced production of L-lactic acid by Lactobacillus thermophilus SRZ50 mutant generated by high-linear energy transfer heavy ion mutagenesis[J]. ENGINEERING IN LIFE SCIENCES,2018,18:626-634.
APA Li, Wen-jian.,Hu, Wei.,Jiang, Ai-lian.,Chen, Ji-hong.,Lu, Dong.,...&Liu, Lu.(2018).Enhanced production of L-lactic acid by Lactobacillus thermophilus SRZ50 mutant generated by high-linear energy transfer heavy ion mutagenesis.ENGINEERING IN LIFE SCIENCES,18,626-634.
MLA Li, Wen-jian,et al."Enhanced production of L-lactic acid by Lactobacillus thermophilus SRZ50 mutant generated by high-linear energy transfer heavy ion mutagenesis".ENGINEERING IN LIFE SCIENCES 18(2018):626-634.

入库方式： OAI收割

来源：近代物理研究所

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