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Strain improvement of Trichoderma viride for increased cellulase production by irradiation of electron and C-12(6+)-ion beams

文献类型：期刊论文

作者Gao, Hongli1; Li, Zhaozhou1; Chen, Xiujin1; Li, Zhili1; Li, Daomin1; Wang, Yao1; Cao, Li1; Hou, Yuze1; Li, Songbiao1; Liang, Jianping2,3
刊名BIOTECHNOLOGY LETTERS
出版日期2016-06-01
卷号38页码:983-989
关键词Cellulase C-12(6+)-ion beam irradiation Electron beam irradiation Mutation Trichoderma viride
ISSN号0141-5492
DOI10.1007/s10529-016-2066-7
英文摘要To improve cellulase production and activity, Trichoderma viride GSICC 62010 was subjected to mutation involving irradiation with an electron beam and subsequently with a C-12(6+)-ion beam. Mutant CIT 626 was the most promising cellulase producer after preliminary and secondary screening. Soluble protein production and cellulase activities were increased mutifold. The optimum temperature, pH and culture time for the maximum cellulase production of the selected mutant were 35 A degrees C, pH 5 and 6 days. The highest cellulase production was obtained using wheat bran. The prepared cellulases from T. viride CIT 626 had twice the hydrolytic performance with sawdust (83 %) than that from the parent strain (42.5 %). Furthermore, molecular studies demonstrated that there were some key mutation sites suggesting that some amino acid changes in the protein caused by base mutations had led to the enhanced cellulase production and activity. Mutagenesis with electron and C-12(6+)-ion beams could be developed as an effective tool for improvement of cellulase producing strains.
WOS关键词CARBON ION-BEAMS
资助项目National Natural Science Foundation of China[U1504330] ; Doctoral Research Initiation Grant from Henan University of Science and Technology[13480030] ; National Student Research Training Program of Ministry of Education of China[201310464007] ; Youth Science Foundation of Henan University of Science and Technology[2013QN021] ; Innovative Capacity Cultivating Foundation in Natural Science of Henan University of Science and Technology[2014ZCX021] ; Key Science and Technology Program of Educational Commission of Henan Province, China[14B550015]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000375566400010
出版者SPRINGER
资助机构National Natural Science Foundation of China ; Doctoral Research Initiation Grant from Henan University of Science and Technology ; National Student Research Training Program of Ministry of Education of China ; Youth Science Foundation of Henan University of Science and Technology ; Innovative Capacity Cultivating Foundation in Natural Science of Henan University of Science and Technology ; Key Science and Technology Program of Educational Commission of Henan Province, China
源URL[http://119.78.100.186/handle/113462/42544]  
专题近代物理研究所_重离子辐照药物研发中心
通讯作者Chen, Xiujin
作者单位1.Henan Univ Sci & Technol, Coll Food & Bioengn, 263 Kaiyuan Ave, Luoyang 471023, Peoples R China
2.Chinese Acad Agr Sci, Lanzhou Inst Anim Sci & Vet Pharmaceut Sci, Lanzhou 730050, Peoples R China
3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730050, Peoples R China
推荐引用方式
GB/T 7714		 Gao, Hongli,Li, Zhaozhou,Chen, Xiujin,et al. Strain improvement of Trichoderma viride for increased cellulase production by irradiation of electron and C-12(6+)-ion beams[J]. BIOTECHNOLOGY LETTERS,2016,38:983-989.
APA Gao, Hongli.,Li, Zhaozhou.,Chen, Xiujin.,Li, Zhili.,Li, Daomin.,...&Liang, Jianping.(2016).Strain improvement of Trichoderma viride for increased cellulase production by irradiation of electron and C-12(6+)-ion beams.BIOTECHNOLOGY LETTERS,38,983-989.
MLA Gao, Hongli,et al."Strain improvement of Trichoderma viride for increased cellulase production by irradiation of electron and C-12(6+)-ion beams".BIOTECHNOLOGY LETTERS 38(2016):983-989.

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来源：近代物理研究所

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