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Enzyme-assisted extraction of naphthodianthrones from Hypericum perforatum L. by C-12(6+)-ion beam-improved cellulases

文献类型：期刊论文

作者Li, Xuehu1; Li, Zhaozhou3; Wang, Xuehong3; Shi, Guangliang2; Bo, Yongheng2; Lu, Xihong1; Shang, Ruofeng3; Tao, Lei2; Liang, Jianping3
刊名SEPARATION AND PURIFICATION TECHNOLOGY
出版日期2012-02-15
卷号86页码:234-241
关键词Enzyme-assisted Extraction Naphthodianthrones C-12(6+)-ion Beam Mutant Screening Cellulase
ISSN号1383-5866
DOI10.1016/j.seppur.2011.11.009
文献子类Article
英文摘要The production of cellulase by Aspergillus niger and Trichoderma viride strains was significantly enhanced via irradiation with a C-12(6+)-ion beam. Successive mutants showed a clearance zone on cellulose Congo red medium and increased cellulase activities. By using these cellulases, enzyme-assisted extractions of the naphthodianthrones hypericin and pseudohypericin from Hypericum perforatum L. were examined for the first time. The strong disruption of plant tissue structure through cellulase maceration was observed with scanning electron microscopy. Optimum conditions for improving the extraction yield of naphthodianthrones with the purified cellulases were as follows: the dried plant powder was treated with a cellulase solution of 150 U/g substrate and incubated at pH 5 for 6 h at 40 degrees C. The extraction yields of purified hypericin and pseudohypericin were up to 166.5 mu g/g and 260.6 mu g/g, respectively, in the cellulase-treated sample, representing higher yields than sonication and Soxhlet extractions. Cellulase-assisted extraction was proven to be highly efficient in the degradation of plant cell-wall polysaccharides and could be used in natural product extraction on a large scale. (C) 2011 Elsevier B.V. All rights reserved.
WOS关键词ST-JOHNS WORT ; FILTER-PAPER ASSAY ; FERMENTATION ; PURIFICATION ; HYDROLYSIS ; DIGESTION ; RELEASE ; MUTANT
资助项目National Key Technologies Research and Development Program of China[2006BAD31B05]
WOS研究方向Engineering
语种英语
WOS记录号WOS:000301757700028
出版者ELSEVIER SCIENCE BV
源URL[http://119.78.100.186/handle/113462/48927]  
专题近代物理研究所_重离子辐照药物研发中心
通讯作者Liang, Jianping
作者单位1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730050, Peoples R China
2.Gansu Agr Univ, Coll Vet Med, Lanzhou 730050, Peoples R China
3.Lanzhou Inst Husb & Pharmaceut Sci CAAS, Minist Agr, Key Lab New Anim Drug Project, Gansu Prov Key Lab Vet Pharmaceut Dev, Lanzhou 730050, Peoples R China
推荐引用方式
GB/T 7714		 Li, Xuehu,Li, Zhaozhou,Wang, Xuehong,et al. Enzyme-assisted extraction of naphthodianthrones from Hypericum perforatum L. by C-12(6+)-ion beam-improved cellulases[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2012,86:234-241.
APA Li, Xuehu.,Li, Zhaozhou.,Wang, Xuehong.,Shi, Guangliang.,Bo, Yongheng.,...&Liang, Jianping.(2012).Enzyme-assisted extraction of naphthodianthrones from Hypericum perforatum L. by C-12(6+)-ion beam-improved cellulases.SEPARATION AND PURIFICATION TECHNOLOGY,86,234-241.
MLA Li, Xuehu,et al."Enzyme-assisted extraction of naphthodianthrones from Hypericum perforatum L. by C-12(6+)-ion beam-improved cellulases".SEPARATION AND PURIFICATION TECHNOLOGY 86(2012):234-241.

入库方式： OAI收割

来源：近代物理研究所

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