Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry
文献类型：期刊论文
| 作者 | Sun, Ruixiang2; Liu, Zheyi3; Wang, Ruimin2; Liu, Jing1; Wang, Fangjun3 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2019-05-01 |
| 卷号 | 18期号:5页码:2185-2194 |
| 关键词 | mass spectrometry chemical isotope labeling intact protein quantification pTOP highest isotopic peak |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/acs.jproteome.9b00071 |
| 英文摘要 | Although thousands of intact proteins have been feasibly identified in recent years, global quantification of intact proteins is still challenging. Herein, we develop a high-throughput strategy for global intact protein quantification based on chemical isotope labeling. The isotope incorporation efficiency is as high as 99.2% for complex intact protein samples extracted from HeLa cells. Further, the pTop 2.0 software is developed for automated quantification of intact proteoforms in a high-throughput manner. The high quantification accuracy and reproducibility of this strategy have been demonstrated for both standard and complex cellular protein samples. A total of 2283 intact proteoforms originated from 660 protein accessions are successfully quantified under anaerobic and aerobic conditions and the differentially expressed proteins are observed to be involved in the important biological processes such as stress response. |
| 资助项目 | National Key R&D Program of China[2016YFF0200504] ; National Natural Science Foundation of China[21675152] ; National Natural Science Foundation of China[91853101] ; National Natural Science Foundation of China[31670837] ; DICP[ZZBS201603] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000467317700023 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/4249]  |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Sun, Ruixiang; Wang, Fangjun |
| 作者单位 | 1.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China 2.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Ruixiang,Liu, Zheyi,Wang, Ruimin,et al. Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry[J]. JOURNAL OF PROTEOME RESEARCH,2019,18(5):2185-2194. |
| APA | Sun, Ruixiang,Liu, Zheyi,Wang, Ruimin,Liu, Jing,&Wang, Fangjun.(2019).Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry.JOURNAL OF PROTEOME RESEARCH,18(5),2185-2194. |
| MLA | Sun, Ruixiang,et al."Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry".JOURNAL OF PROTEOME RESEARCH 18.5(2019):2185-2194. |
入库方式： OAI收割
来源：计算技术研究所
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